BioLector XT

Written by Sophie Lindsey on September 25, 2023. Posted in Products.

Beckman Coulter
BioLector XT

Microbioreactor

Multiple processe
Enhanced PIDS Technology
Real data down to 10nm

$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

BioLector XT microbioreactor accelerates your bioprocess development

High-throughput microbioreactor enables real-time evaluation of biomass, fluorescence, pH, dissolved oxygen in the liquid phase (DO), and other key cultivation parameters for aerobes and anaerobes.

Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with online, pre-calibrated optical sensors. Disposable 48 well MTPs enable online measurement of cultivation parameters, while patented microfluidic technology supports simultaneous pH control and feeding. The optional microfluidic module eliminates manual liquid handling—no tubing or pipetting required, as everything is part of the beta-radiated ready-to-use plate.

Meritics are proud to partner with Beckman Coulter Life Sciences by hosting the UK demonstration unit of the BioLector XT microbioreactor at our premises. Extensive experience within the UK biologics sector and highly rated application and laboratory personnel makes Meritics the ideal partner to showcase the BioLector XT microbioreactor and its real-world applications.

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Key Features
Innovative New Gassing Head
- Enables fed-batch experiments under anaerobic conditions
- Gassing with O2 within a range of 1% – 100% and with CO2 within 1% – 12 %
- Reduces gas consumption to a few mL/minute
- Optional humidification of gases reduces evaporation
Optional Microfluidic Module
- Unleashes full potential of the BioLector XT
- Complements online monitoring function with well-specific pH regulation/feeding
- Enables use of 2 reservoir wells per 4 cultivation wells
- Microvalves allot liquids at nanoliter-scale
“Plug-and-play” Plate Design
- Real-time kinetics out of 48/32 parallel cultivations
- Customisable feeding strategies (batch, fed-batch, bolus, continuous)*
- Control of pH on-the-plate with pre-calibrated optical sensors*
- Small working volume (800 – 2400 μL)
*Functionality requires optional microfluidic module
Intelligent BioLection Software
- Intuitive user interface supports multi-user environments
- Free programming of all control parameters
- Open system enables live data downloads
- Fast processor ensures rapid download of experiment data

Technical Specs

Volume	800 – 2400 µL
pH Range	pH 4 – 7.5 (depending on plate)
Scattered Light Measurement	Resolution > 50 NTU, at densities higher than 500 NTU: 10 % of measured value.
Height	522 mm(20.6 in)
Width	797 mm(31.4 in)
Depth	520 mm(20.5 in)
Temperature range	10 – 50 °C (minimum temperature 8 °C below ambient temperature)
DO Range	0 – 100% oxygen saturation (100% corresponding to the DO level reached while gassing with 100% O2 without O2 consumption)
Wavelengths	365 nm–800 nm
Weight	61 kg(134.5 lb)

Accessories

Microfluidic Module

The microfluidic (MF) module for the BioLector XT microbioreactor allows you to run up to 32 highly flexible, pH-controlled fed-batch cultivations in microscale during one cultivation experiment run.

Anaerobic Clutivation

The anaerobic module for the BioLector XT microbioreactor enables strict anaerobic fermentation processes combined with a controlled, low nitrogen gas flow rate.

CO2 Up Regulation

With the CO2 up-regulation module the microbioreactor continuously measures the CO2 level inside the chamber and automatically regulates the flow of CO2 into the chamber.

LED Filter

With the LED / filter module, the microbioreactor can measure additional fluorescences. An LED unit and two optical filter glasses are installed inside the BioLector microbioreactor.

O2 Down Regulation

With the O2 down-regulation module the microbioreactor continuously measures the oxygen level inside the chamber and automatically regulates the flow of nitrogen into the chamber.

O2 Up Regulation

With the O2 up-regulation module the microbioreactor continuously measures the oxygen level inside the chamber and automatically regulates the flow of oxygen into the chamber.

There is more information on all the accessories on the Beckman Coulter website
Applications

Yeast

E. coli

Probiotic Bacteria

Plant Cells