Orflo
Moxi V
Automated Cell Counter
- True Cell Viability Counts
- Precision Sizing
- Highly Accurate
- Rapid Assays
Overview
The Moxi V Automated Cell Counter provides a combination of volumetric cell sizing (Particle Sizer) with simultaneous fluorescence (Cell Analyzer) to provide the most accurate cell counts, size, and viability in the industry. Specifically, the Moxi V employs the Coulter Principle to precisely measure the volumetric particle size of each particle for exact size measurements down to 3um in diameter (14fL volume), easily distinguishing between cells and debris. The system is also equipped with a 532nm laser and a 561nm/LP detection channel for robust cell viability analysis. Propidium Iodide (PI)-stained dead cells measure 50-100 times brighter fluorescence on the system than do live cells, removing the ambiguity associated with traditional Trypan Blue viability assessments. For each test, these size and viability measurements are applied, individually, to up to 23,000 cells in a matter of a few seconds. This ensures the highest level of precision and statistical robustness. As the fluidic volume is precisely metered as well, the particle counts are presented as an exact cell concentration.
The Moxi V employs a patented, single-use, microfluidic flow cell. The flow cells eliminate the hassle of traditional Cell Analyzers and Coulter Counters, eliminating the need for cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes. The Moxi V uses very little sample volume, 60ul, allowing you to conserve your precious, potentially expensive, sample (e.g. stem cells). Cell concentrations as low as 10 cells/ul are possible, typically requiring just 5ul of cell sample diluted in 55ul of PBS.
The Moxi V™ system comes standard with an ultra-intuitive, plug-and-play interface with free OS updates for as long as you own the instrument. No prior Cell Analysis experience is required – you simply just plug and play.
Some Key Features of the Moxi V include:
- True Cell Viability Counts – 50-100x more sensitive than vision counters.
- Precision Sizing – Uses the Coulter Principle to get precise cell volumes with CVs less than 3% and no need for triplicates.
- Highly Accurate – Accurate counts for smaller cells down to 3um (i.e. nuclei, RBCs). Uniquely accurate at low cell concentrations down to 10 cells per ul.
- Rapid Assays – Offers a less than 15 second test that counts up to 23,000 cells compared to 200-300 counts on most vision counters.
Technical Specs
|
Id
|
MXV102 |
|
Included accessories
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USB power cord, US style USB power adapter, and Type S+ cassette pack |
|
AC power type
|
110 VAC |
|
Applications
|
Gold Standard Cell Count, Cell Size, and Viability |
|
Battery Type
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Rechargeable 3.7 V, 7500 mAh lithium ion |
|
Cassette types
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Type S+ |
|
Cell Particle Concentration Range
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10,000 – 5,000 cells/mL Type S+ |
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Cell types tested
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HEK-293 |
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Data output formats
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FCS 3.1, screen shots (.bmp), CSV |
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Data storage capasity
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4Gb |
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Display resolution
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800 x 480 colour touchscreen |
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Excitation wavelengths
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532nm |
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In British units
|
8 lbs |
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Intended use statement
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For Research Use Only. Product is not for use in diagnostic procedures |
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Laser colour
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Green |
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Measurable dynamic range
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3 – 27 microns Type Type S+ |
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Measurement time
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10 seconds Type S+ |
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MPI cell health ratio
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Yes (Size histogram only) |
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Number of detection channels flow parameters
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2 colour, 1 size, 1 forward extinction |
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Number of PMTs
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1 |
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Optical detection range
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561nm/LP (e.g. PI) |
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Particle size detection method
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Impedimetric (Coulter Principle) |
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Pre-programmed tests
|
Gold Standard Cell Count, Cell Size, and Viability |
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Sample type
|
Cell Preparations |
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Sample volume
|
60 µL |
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Supported connectivity
|
USB on-the-go |
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Useable cell volume
|
14 – 10,306 fL Type S+ |
|
Weight
|
3.6 kg |
How it works
The operating principle behind the Moxi V is a unique combination of Coulter-Principle cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser with a 561nm/LP (e.g. PI) emission filter. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is automatically performed by the system but can be easily adjusted/tuned by the user. The resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analysed.
Data
Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis package. The actual Moxi V screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi V and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.
Not sure if it’s the right instrument?
No worries, send us a sample and we will test it for you
Send a sampleOutstanding service
I have been using Meritics for a number of years and they always provide outstanding service and support.
They have a rapid response turnarounds and are very knowledgeable of the techniques and products they provide.
LeanneB-375
Good service, very helpful
We use Meritics for the PM service and installation of all our FlowCams . Megan has been coming to our site for a number of years. She is very efficient in her work, but most of all Megan is very helpful. We have had issues with one of instruments after the install and Megan did her utmost to try and solve it while on site, even though it wasn’t solved Megan has been in contact with the supplier of the instrument to try and solve this problem for us.
SophieH-2201
Job done in a friendly manner
Booking engineer visits is always easy. David Lowe, Meritics engineer is always very friendly.
ThierryN
Service/calibration visit
Excellent service, arrived on time, polite, friendly and informative.
NathamM-270
5 Stars
Excellent service from an excellent company. Would recommend them to anyone.
BarryH-480
Good Service
David, our service engineer as usual was good at communicating and setting up the recent service appointment, he arrived on time, did the service promptly and informed me of any problems. Friendly and helpful
TomA-446
Superb and prompt service
I have used this company through the laboratory I work for frequently. We specialise in soil analysis, and Meritics were chosen as they have extensive knowledge of the instruments we use. We have several laser particle diffraction analysers with a service contract, and service is always very prompt and meticulous….
JamesG-2483
Good experience over two years
Meritics have covered the annual servicing of our Multisizer 4e instrument since we purchased it from them just over two years ago. The application scientist who performed the servicing was friendly, knowledgeable, and extremely helpful, happily answering a barrage of questions about how best to use and maintain the instrument. All my interactions with Meritics staff have been positive. I would happily recommend them.
RajG-62
Fully committed to customer service
Service second to none, both units serviced and all questions answers by engineer while on site.
RichardA-786
Highly recommend Meritics for equipment purchases and aftercare services
After owning one of our instruments for over 8 Years which was purchased from Meritics, it suffered its first breakdown. Upon contacting Meritics, I was instantly put through to an engineer who was very helpful. Upon inspection of the manual, it was decided that it would be more cost effective to have a Meritics engineer visit. The Meritics staff were very communicative and kept me up to date.
Excellent Service
Lovely, friendly and extremely helpful. David and his team are always on call with advise and are very easy to work with and are always happy to help.
NaomiT-64
Service of lab equipment
Excellent service. Friendly and professional service was provided in a timely manner to the expected standard.
NathanM-270
Excellent
Excellent communication. Updates given well in advance. Very polite staff and engineers.
NathanM-270
Multisizer 4e install at pharmaceutical company
2 people helped with the install of a Multisizer 4e. They were both very professional and friendly, answering any questions that I had. The installation was quick and the attention to good documentation practice was exceptional.
Chloel-9
Service of Beckman Coulter instruments
Always a pleasure to welcome the Meritics service engineer, who is knowledgeable, personable and extremely competent.
NeilT-394
Professional
Friendly and collaborative staff, provided a very good service.
SamK-403
Professional, Customer-oriented, fast service
We had issues with one of the aperture tubes provided by Meritics on a Friday afternoon, while very pressed for a review deadline. Meritics handled our inquiries very fast and professionally and helped us out by shipping us their spare tube to use in the mean time free of charge, while we waited for our new tube to arrive. Communication was very pleasant.
VeerleB
Very helpful and prompt
I emailed for some technical help and also to get some spares and both the engineer (David) and purchasing (Sophie) were very prompt to respond and helpful. We do have a service contract but I appreciated being able to email the engineer directly.
AlexH-474
Excellent Service
Used Meritics to conduct particle size distribution by laser diffraction. Service as last time, is very quick, well priced and professional.
I would highly recommend
GavinR-101
Excellent and Friendly Service
Always a pleasure to deal with the people at Meritics Ltd. They understand the importance of our work and the fact that our research projects have deadlines that need to be met. Consequently, problems are dealt with very quickly and professionally and they are willing to go that one step further to make sure their supply chain problems don’t become our problems. Thank you to the team for their support. It is greatly appreciated.
JanetH-255