Rapid quality control of antibodies using Affinité’s simple P4SPRTM
The quality control of biopharmaceuticals such as antibodies must be performed to ensure quality and safety. For instance, the quality of antibodies is affected by process parameters such as pH, temperature, CO2, and cell culture metabolites [1]. Not only should these biopharmaceuticals be characterized by standard physicochemical methods such as mass spectrometry, but their biological activities must also be investigated as well. This has traditionally been done using endpoint assays such as Enzyme-Linked Immunosorbent Assays (ELISA). However, these assays do not provide kinetic and affinity data. An alternative method is Surface Plasmon Resonance (SPR). SPR is a powerful technique to characterize proteins because it is a label free technique that enables real-time assessment of protein interactions, and it does not require much sample preparation.
