Characterising Biological Aggregates
Characterisation of protein aggregates involves analysing size, morphology, and distribution using techniques like dynamic light scattering (DLS), Surface Plasmon Resonance (SPR) and Flow Imaging Microscopy (FIM). This helps understand aggregation mechanisms, assess stability, and ensure safety and efficacy in pharmaceuticals, reducing immunogenicity risks and optimising formulation and storage conditions.
Dynamic light scattering (DLS)
Dynamic light scattering (DLS) characterises protein aggregates by measuring their size distribution in solution, providing insights into aggregation state, stability, and polydispersity, crucial for pharmaceutical formulation and quality control.
Surface plasmon resonance (SPR)
Surface plasmon resonance (SPR) characterises protein aggregates by measuring binding interactions and kinetics on sensor surfaces, providing insights into aggregation behavior, affinity, and stability, crucial for therapeutic protein development and quality assurance.
Flow Imaging Microscopy (FIM)
Flow Imaging Microscopy (FIM) characterises protein aggregates by capturing high-resolution images in a flowing sample, allowing precise analysis of size, shape, and concentration, essential for assessing protein formulation stability and quality.
