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Orflo
Moxi GO II

Mini Automated Cell Analyzer

  • Small footprint enables portability and convenience.
  • Fast (<10 sec) test times and no warm-up periods ensure rapid results.
  • Intuitive, touchscreen design for ease of use.
  • No cleaning or maintenance required.

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Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11

Next Generation Coulter-Principle Cell Analyser.

The Orflo Moxi GO II  Cell Analyser is the best solution for cell counting due to its precision, speed, and ease of use. Combining Coulter Principle-based sizing with fluorescence-based viability, it offers highly accurate and reproducible results. Its intuitive interface and automated processes ensure rapid and user-friendly operation, making it ideal for both novice and experienced users. The Moxi GO II’s compact design and high-throughput capabilities make it perfect for various applications, including research labs and biotech industries, providing consistent and reliable cell analysis.

Overview

Combines the Coulter Principle (for highly-accurate cell counts and exact, volumetric cell sizing) with a 488nm laser and two PMT fluorescence detection channels (for cell health assays, robust CAR-T monitoring, cellular response profiling, and immuno-profiling).

The Moxi GO II’s new Auto-Gating feature will analyse results in an accurate, repeatable way to provide the most consistent results — presented in a simplified new Data Summary page. The new Batch Mode feature allows you to run multiple tests of the same sample type. It will auto-find live and dead cell populations, eliminating user-to-user variability.

Technical Specs

Id

MXG102

Included accessories

USB power cord, US style USB power adapter, and Type S+ cassette pack

AC power type

110 VAC

Applications

Mulitplexed Bead ELISA’s
In Cell Westerrns
In Cell Protein Quant
GFP
Gold Standard Cell Count and Viability
Mito Potential
ROS
Phagocytosis

Battery Type

Rechargeable 3.7 V, 4400 mAh lithium ion

Cassette types

Type S+

Cell Particle Concentration Range

5,000 – 1,000,000 cells/mL Type S+

Cell types tested

HEK-293
HeLa
PC12
CD3+T
CHO-K1
Cos-7
HepG2
Hybridoma
Jurkat E6-1
K562
MCF7
Mesenchymal SC
Monocyte
Mouse ESC
NIH 3T3
PBMC (cultured)
Red Blood Cells (RBC)
L5178y
C. albicans (Yeast)
S. cerevisiae Vin 13 (Yeast)
S. cerevisiae X5 (Yeast)
Wine Yeast (natural fermentaion)
S.cerevisiae (Baker’s Yeast
Safale US-05 Yeast

Data output formats

FCS 3.1, screen shots (.bmp), CSV

Data storage capasity

4Gb

Display resolution

800 x 480 color touchscreen

Excitation wavelengths

488nm

In British units

10 lbs

Intended use statement

For Research Use Only. Product is not for use in diagnostic procedures

Laser colour

Blue

Measurable dynamic range

3 – 27 microns Type Type S+
4 – 35 microns Type MF-M

Measurement time

10 seconds Type S+

MPI cell health ratio

Yes (Size histogram only)

Number of detection channels flow parameters

2 color, 1 size, 1 forward extinction

Number of PMTs

2

Optical detection range

525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP)

Particle size detection method

Impedimetric (Coulter Principle)

Platform

Open platform: 561nm/LP (PI, PE, DS Red, Sytox Orange, 7 AAD, Nile Red, Rhodamine Red, Sun Coast Yellow, PE/Cy5), 525/45nm (FITC, GFP, Alexa Fluor 488nm, Calcein)

Pre-programmed tests

Mulitplexed Bead ELISA’s
In Cell Westerrns
In Cell Protein Quant
RFP
Gold Standard Cell Count and Viability
Mito Potential
ROS
Phagocytosis

Resolution histogram bins

1000

Sample type

Beads
Cell Preparations

Sample volume

60 µL

Supported connectivity

USB on-the-go

Useable cell volume

14 – 10,306 fL Type S+
14 – 22,449 fL Type MF-M

Weight

4.53 kg

How it works

The operating principle behind the Moxi GO II Cell Analysers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 488nm (MXG102) solid state diode laser with and with the following emission filters – 525/45nm (e.g. FITC, GFP, Alexa 488) and 561nm/LP (e.g. PE, RFP). Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analysed.

Step 1: Select desired app, insert the cassette and close the doors.


Step 2: Once auto-alignment is complete, open the top door and pipette 60μl of labelled sample into the cassette.
Step 3: Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi GO, and load Sample 2.
Data

Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi GO and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.

Applications

Apoptosis Monitoring

Transfection Monitoring

Yeast Monitoring in Brewing

Immuno-phenotyping

Mitochondrial Membrane

Reactive Oxygen Species

Not sure if it’s the right instrument?

No worries, send us a sample and we will test it for you

Send a sample

Outstanding service

I have been using Meritics for a number of years and they always provide outstanding service and support.
They have a rapid response turnarounds and are very knowledgeable of the techniques and products they provide.

LeanneB-375

Good service, very helpful

We use Meritics for the PM service and installation of all our FlowCams . Megan has been coming to our site for a number of years. She is very efficient in her work, but most of all Megan is very helpful. We have had issues with one of instruments after the install and Megan did her utmost to try and solve it while on site, even though it wasn’t solved Megan has been in contact with the supplier of the instrument to try and solve this problem for us.

SophieH-2201

Job done in a friendly manner

Booking engineer visits is always easy. David Lowe, Meritics engineer is always very friendly.

ThierryN

Service/calibration visit

Excellent service, arrived on time, polite, friendly and informative.

NathamM-270

5 Stars

Excellent service from an excellent company. Would recommend them to anyone.

BarryH-480

Good Service

David, our service engineer as usual was good at communicating and setting up the recent service appointment, he arrived on time, did the service promptly and informed me of any problems. Friendly and helpful

TomA-446

Superb and prompt service

I have used this company through the laboratory I work for frequently. We specialise in soil analysis, and Meritics were chosen as they have extensive knowledge of the instruments we use. We have several laser particle diffraction analysers with a service contract, and service is always very prompt and meticulous….

JamesG-2483

Good experience over two years

Meritics have covered the annual servicing of our Multisizer 4e instrument since we purchased it from them just over two years ago. The application scientist who performed the servicing was friendly, knowledgeable, and extremely helpful, happily answering a barrage of questions about how best to use and maintain the instrument. All my interactions with Meritics staff have been positive. I would happily recommend them.

RajG-62

Fully committed to customer service

Service second to none, both units serviced and all questions answers by engineer while on site.

RichardA-786

Highly recommend Meritics for equipment purchases and aftercare services

After owning one of our instruments for over 8 Years which was purchased from Meritics, it suffered its first breakdown. Upon contacting Meritics, I was instantly put through to an engineer who was very helpful. Upon inspection of the manual, it was decided that it would be more cost effective to have a Meritics engineer visit. The Meritics staff were very communicative and kept me up to date.

Excellent Service

Lovely, friendly and extremely helpful. David and his team are always on call with advise and are very easy to work with and are always happy to help.

NaomiT-64

Service of lab equipment

Excellent service. Friendly and professional service was provided in a timely manner to the expected standard.

NathanM-270

Excellent

Excellent communication. Updates given well in advance. Very polite staff and engineers.

NathanM-270

Multisizer 4e install at pharmaceutical company

2 people helped with the install of a Multisizer 4e. They were both very professional and friendly, answering any questions that I had. The installation was quick and the attention to good documentation practice was exceptional.

Chloel-9

Service of Beckman Coulter instruments

Always a pleasure to welcome the Meritics service engineer, who is knowledgeable, personable and extremely competent.

NeilT-394

Professional

Friendly and collaborative staff, provided a very good service.

SamK-403

Professional, Customer-oriented, fast service

We had issues with one of the aperture tubes provided by Meritics on a Friday afternoon, while very pressed for a review deadline. Meritics handled our inquiries very fast and professionally and helped us out by shipping us their spare tube to use in the mean time free of charge, while we waited for our new tube to arrive. Communication was very pleasant.

VeerleB

Very helpful and prompt

I emailed for some technical help and also to get some spares and both the engineer (David) and purchasing (Sophie) were very prompt to respond and helpful. We do have a service contract but I appreciated being able to email the engineer directly.

AlexH-474

Excellent Service

Used Meritics to conduct particle size distribution by laser diffraction. Service as last time, is very quick, well priced and professional.

I would highly recommend

GavinR-101

Excellent and Friendly Service

Always a pleasure to deal with the people at Meritics Ltd. They understand the importance of our work and the fact that our research projects have deadlines that need to be met. Consequently, problems are dealt with very quickly and professionally and they are willing to go that one step further to make sure their supply chain problems don’t become our problems. Thank you to the team for their support. It is greatly appreciated.

JanetH-255