The ViewSizer 3000 uses the latest advances in nanoparticle tracking analysis (NTA) to accurately determine particle properties!

Size Measurement Range: 10 nm to 15 microns depending on sample

The ViewSizer determines particle size and size distribution details by multi-laser nanoparticle tracking analysis (NTA). Multiple lasers enable analysis of a broad range of sizes in the same sample.

Concentration Measurement Range: 1E5 to 1E9 particles/mL

NTA can be used to count particles in the measurement volume. A patented method corrects for the effect of particle size on effective measurement volume.

No cross-contamination

The sample cell can be completely removed and dissassembled for complete cleaning. Disassembly, cleaning, and reassembly is faster than rinsing a flow cell. Furthermore, multiple cells can be used for faster throughput or assigned to various groups in a shared (core) facility.

Featured Publications for the ViewSizer 3000:

Videodrop films in real time biological nanoparticles between 80 nm & 500 nm and provides a microscopic view up to 10µm in a drop. The compact device, assisted by a dedicated software, allows the manipulation to be very easy to perform. No labeling is necessary on the analyzed sample. It is possible to work on unpurified solutions and on little volumes (5µL) with a concentration range of 10^8 to 10^10 particles/ml.

Key Benefits:

• Precision & Accuracy: Delivers reliable data for confident research and applications.
• High-Resolution Imaging: Offers detailed insights into nanoparticle morphology and behaviour.
• Real-Time Analysis: Monitors particle dynamics in real-time, crucial for virology and other fields.
• Versatility: Suitable for diverse applications across pharmaceuticals, biotechnology, and material science.
• User-Friendly Interface: Simplifies operation, increasing productivity for all users.
• Cost-Effective: Provides a robust, low-maintenance solution with high return on investment.
• Advanced Software: Facilitates comprehensive data analysis and visualisation.
• High-Throughput: Efficiently processes multiple samples for large-scale research and quality control.

Choose VideoDrop for unparalleled nanoparticle analysis, enhancing your research and applications with precision and efficiency.

The Moxi V Automated Cell Counter provides a combination of volumetric cell sizing (Particle Sizer) with simultaneous fluorescence (Cell Analyzer) to provide the most accurate cell counts, size, and viability in the industry. Specifically, the Moxi V employs the Coulter Principle to precisely measure the volumetric particle size of each particle for exact size measurements down to 3um in diameter (14fL volume), easily distinguishing between cells and debris. The system is also equipped with a 532nm laser and a 561nm/LP detection channel for robust cell viability analysis. Propidium Iodide (PI)-stained dead cells measure 50-100 times brighter fluorescence on the system than do live cells, removing the ambiguity associated with traditional Trypan Blue viability assessments. For each test, these size and viability measurements are applied, individually, to up to 23,000 cells in a matter of a few seconds. This ensures the highest level of precision and statistical robustness. As the fluidic volume is precisely metered as well, the particle counts are presented as an exact cell concentration.

The Moxi V employs a patented, single-use, microfluidic flow cell. The flow cells eliminate the hassle of traditional Cell Analyzers and Coulter Counters, eliminating the need for cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes. The Moxi V uses very little sample volume, 60ul, allowing you to conserve your precious, potentially expensive, sample (e.g. stem cells). Cell concentrations as low as 10 cells/ul are possible, typically requiring just 5ul of cell sample diluted in 55ul of PBS.

The Moxi V™ system comes standard with an ultra-intuitive, plug-and-play interface with free OS updates for as long as you own the instrument. No prior Cell Analysis experience is required – you simply just plug and play.

Some Key Features of the Moxi V include:

• True Cell Viability Counts – 50-100x more sensitive than vision counters.
• Precision Sizing – Uses the Coulter Principle to get precise cell volumes with CVs less than 3% and no need for triplicates.
• Highly Accurate – Accurate counts for smaller cells down to 3um (i.e. nuclei, RBCs). Uniquely accurate at low cell concentrations down to 10 cells per ul.
• Rapid Assays – Offers a less than 15 second test that counts up to 23,000 cells compared to 200-300 counts on most vision counters.

The operating principle behind the Moxi V is a unique combination of Coulter-Principle cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser with a 561nm/LP (e.g. PI) emission filter. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is automatically performed by the system but can be easily adjusted/tuned by the user. The resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analysed.

Step 1:
Select desired app, insert the cassette and close the doors.

Step 2:
Once auto-alignment is complete, open the top door and pipette 60μl of labelled sample into the cassette.

Step 3:
Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi V, and load Sample 2.

Data

Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis package. The actual Moxi V screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi V and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.

Combines the Coulter Principle (for highly-accurate cell counts and exact, volumetric cell sizing) with a 488nm laser and two PMT fluorescence detection channels (for cell health assays, robust CAR-T monitoring, cellular response profiling, and immuno-profiling).

The Moxi GO II’s new Auto-Gating feature will analyse results in an accurate, repeatable way to provide the most consistent results — presented in a simplified new Data Summary page. The new Batch Mode feature allows you to run multiple tests of the same sample type. It will auto-find live and dead cell populations, eliminating user-to-user variability.

The operating principle behind the Moxi GO II Cell Analysers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 488nm (MXG102) solid state diode laser with and with the following emission filters – 525/45nm (e.g. FITC, GFP, Alexa 488) and 561nm/LP (e.g. PE, RFP). Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analysed.

Step 1:
Select desired app, insert the cassette and close the doors.

Step 2:
Once auto-alignment is complete, open the top door and pipette 60μl of labelled sample into the cassette.

Step 3:
Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi GO, and load Sample 2.

Data

Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi GO and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.

Improve downstream results with better quality control.

Moxi Z is the only automated cell counter that combines the Coulter Principle typically used in high-end cell counters with a patented thin-film sensor technology to allow for highly accurate (> 95%) and repeatable particle counting and sizing for a broad range of cell types – from mammalian cells to cells as small as wine yeast and more. Since today’s workflows demand accurate quality control of samples, determining cell counts precisely has a significant impact on outcomes and downstream costs.

Produce repeatable, precise cell counting with >95% accuracy in just 8 seconds.

This ultra-small instrument uses patented microfluidic thin-film cassettes that enable automatic load and measure operation. The resulting single cell volumetric measurements are completely technique-independent. It provides the ideal alternative to the tedious manual counting associated with cytometers, or the inaccurate results associated with image-based automated cell counters (typical accuracies of 75-80%).

Assess cell culture health without reagents or dyes.

Moxi Z also automatically reports a unique cell health assessment index – MPI (Moxi Population Index) – without the need for reagents or dyes.MPI is a ratio of the cell population of interest relative to the entire particle distribution in that sample, factoring in dead cells that have shrunken or broken apart as well as other debris and contaminants in the sample. For monodisperse mammalian cultures this is closely correlated to the overall health of the sample.In the case of mixed cell populations, it’s provides an assessment of the relative fraction of the largest sized population in the sample. Unlike staining-based viability methods that focus on the uptake by dead cells and do not account for contaminants and other debris, MPI provides insight on the primary population of interest (based on size) and its relationship to ALL other particles in the sample.

Measure the smallest cells at very high concentrations.

Using the new Type S cassette, you can now accurately measure the smallest cells of any automated cell counter (down to 3um average diameter) at concentrations of up to 2.5e+6/ml. This means the new Type S cassette can measure Yeast (including wine yeast) and non-spherical particles down to 14 fL in volume.

Results in just 8 seconds

• Ultra-small and ultra-easy to use (no manual counting, no focusing of any kind)
• Complete, high-resolution cell size/count histogram
• Handles “de-aggregation” and coincidence events
• Post-processing/analysis of count distributions with user-adjustable regions/gates
• Assess cell health (MPI) without the need for reagents like Trypan blue

Electronic current is passed through a small hole (Cell Sensing Zone) in a thin-film membrane. Cells flow substantially single file through the CSZ causing momentary increases in measured voltage that are directly proportional to cell or particle volume. Thousands of cells are measured during a single test and the size of each cell is plotted and saved in histogram format. Total volumetric counts are determined by precisely measuring the
volume of fluid being analysed.

Finished! Automatic, hands-free measurement in just 8 seconds.

Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into MoxiZ, and load Sample 2.

Data

Data For each measurement, a complete histogram is displayed as well as cell count, average cell size and Moxi Population Index. The raw waveform from the most recent cell count, containing each cell spike, is also stored on the Moxi Z and can be both viewed on the Moxi Z and uploaded to the PC for additional analysis. The Moxi Z also performs sophisticated curve fitting analysis to more accurately determine cell counts in the event of overlapping populations of cells (or debris), significantly improving the accuracy of the volumetric counts produced by the Moxi Z. Data for 500 samples may be stored on the unit. All data can be transferred to a PC/Mac via Bluetooth. No special software is required for the PC since date files are Excel®-compatible. Sophisticated PC/Mac compatible analys software is included with each Moxi Z. Cell health may be assessed by comparing differences in histograms from culture to culture.

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