DeepSizer 300

Written by Sophie Lindsey on October 16, 2025. Posted in Products.

Bettersize Instruments
DeepSizer 300

Submersible Particle Size Analyser

Measures particle sizes from 0.1 to 2000µm
Measures concentrations from 0.001 to 100 g/L
Real-time sediment monitoring in rivers, lakes, estuaries, and coastal waters

$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

YOUR ESSENTIAL TOOL FOR SEDIMENT INSIGHT

The DeepSizer 300 is a submersible particle size analyzer for real-time sediment monitoring in rivers, lakes, estuaries, and coastal waters. It measures concentrations from 0.001 to 100 g/L and particle sizes from 0.1 to 2000 μm with high accuracy.

Powered by six core technologies—including two patented innovations, Differential Path and Adaptive Path Technologies—it adapts to diverse conditions and remains reliable even in extreme environments.

DeepSizer 300 overcomes the risks, delays, inaccuracies, and maintenance burdens of traditional sediment analysis. It delivers trusted insights for sediment studies, ecological monitoring, water-quality assessment, and hydraulic engineering— supporting resilient, sustainable water systems.

Safer In-situ
Measurement

Challenge: Traditional sampling is labor-intensive, time-consuming, and exposes personnel to potential
health and safety risks.

Solution: DeepSizer 300 enables direct in-situ measurement, cutting sampling risks and providing
reliable, continuous data for ecosystems, pollutant tracking, and water safety.

Real-time Sediment Monitoring

Challenge: Conventional methods are too slow to track short-lived spikes in sediment concentration during
floods and typhoons.

Solution: DeepSizer 300 provides real-time measurements of concentration and PSD, capturing
critical flood events to support early warning and protect communities.

Accurate Results in High Turbidity

Challenge: Common optical and acoustic methods lose accuracy in highly turbid waters, limiting their reliability for sediment management.

Solution: DeepSizer 300 combines adaptive sediment concentration measurement with advanced multiplescattering correction, ensuring accurate results even under extreme sediment loads.

Reliable Long-term Field Deployment

Challenge: Extended field campaigns often suffer from window fouling, biofilm growth, and sediment deposits, leading to poor data quality and high maintenance costs.

Solution: DeepSizer 300 features automatic window cleaning and intelligent background correction, enabling long-term unattended operation with high-quality data.

Key Features
- Measures a broad concentration from 0.001 to 100 g/L and particle sizes from 0.1 to 2000 µm, delivering high-precision data across diverse water bodies.
- Measures additional parameters: depth, temperature, and conductivity.
- 80-detector array: detects light signals from 0.0163° to 42°.
- Patented core technologies: incorporate Differential Path Technology and Adaptive Path Technology, ensuring measurement accuracy and superior environmental adaptability.
- Automatic optical window cleaning system: removes deposits and contaminants from optical windows, keeping the instrument in optimal condition.
- Offers three transmission modes—wired, wireless, and offline—to adapt to diverse monitoring scenarios.

Technical Specs

Parameters Measured	Particle size distribution, total mass concentration, depth, temperature, conductivity
Concentration range	0.001 – 100 g/L ( kg/m³))
Particle size analysis range	0.1 – 2000 μm *
Particle size accuracy	≤ 2% *
Particle size repeatability	≤ 2% *
Depth sensor	0 – 200 m
Temperature sensor	-20 – 85°C
Laser source	10 mW, 635 nm
Detector	80 detectors
Measuring angle range	0.0163 – 42°
Optical path length range	0.5 – 40 mm
Depth rating	200 m
Protection level	IP 68
Automatic optical window cleaning	Available, programmable cleaning
Transmission modes	Wired / Wireless (4G) / Offline
Battery runtime	24 hours
Memory capacity	16 GB
Operating temperature	0 – 55°C
Instrument dimensions (L x W x H)	970 x 189 x 189 mm
Weight	20 kg
Computer interface	At least one high-speed USB 2.0 or USB 3.0 port required
Operating system	Windows 10 or above
Hardware specification	Intel Core i5 Processor, 8GB RAM, 512 GB SSD, 1920 x 1080 (Full HD)

*Sample dependent

Applications

Extreme Hydrological Conditions

Suspended Sediment Concentration

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LS 13 320 XR

Written by amanda-meritics on February 8, 2024. Posted in Home page favourites, Products. 2 Comments on LS 13 320 XR

Beckman Coulter
LS 13 320 XR

Laser Diffraction Particle Size Analyser

Expanded measurement range 10 nm – 3,500 µm
Enhanced PIDS Technology
Real data down to 10nm

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$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

Beckman Coulter LS 13 320 XR Particle Size Analyser

For big improvements that help you spot small differences.

The LS 13 320 XR Particle Size Analyser offers best-in-class particle size distribution data from advanced PIDS technology,* which enables high-resolution measurements and an expanded dynamic range. Like the LS 13 320, the XR particle size analyser provides fast, accurate results, and helps you streamline workflows to optimize efficiency. Some big improvements help you reliably spot small differences that can have a huge impact on your particle analysis data.

Direct measurement range from 10 nm – 3,500 µm
Automatically highlights pass/fail results for faster quality control
Enhanced software that simplifies method creation for standardised measurements
New control standards to adequately verify instrument/module performance

The LS 13 320 XR Particle Size Analyser is an easy-to-use laser diffraction analyser that yields fast, reliable particle size analysis data for dry and aqueous and non-aqueous samples.

Key Features
Spot Small Differences
- Particle Size Analyser with expanded measurement range: 10 nm – 3,500 µm
- Laser diffraction plus advanced Polarization Intensity Differential Scattering (PIDS) technology enable high-resolution measurement & reporting of real data down to 10 nm
- Provides accurate, reliable detection of multiple particle sizes in a single sample
Easy-to-use Software
- ADAPT Software features automatic pass/fail check
- Pre-configured methods deliver results with 3 clicks or less
- Simplifies analyser operation by experts & novice users alike
- 1-click overlay with historical data
- Intuitive user diagnostics keep you informed during sampling
- Simplified method creation for standardised measurements
ADAPT Software enables 21 CFR Part 11
- Customisable security system to meet diverse needs
- Choose from 4 security levels
- High-security configuration supports 21 CFR Part 11
PIDS Technology* for Direct Detection of 10 nm Particles
- 3 light wavelengths (450, 600, & 900 nm) irradiate samples with vertical & horizontal polarized light
- Analyser measures scattered light from samples over a range of angles
- Differences between horizontally & vertically radiated light for each wavelength yield high-resolution particle size distribution data

Technical Specs

Technology	Low-angle forward light scattering with additional PIDS(Polarization Intensity Differential Scattering) Technology. Analysis of vertical and horizontal polarized light at six different angles using three additional wavelengths. Full implementation of both Fraunhofer and Mie Theories.
Light Source	Diffraction: Laser Diode (785 nm) PIDS: Tungsten lamp with high-quality band-pass filters (475, 613 and 900 nm)
Particle size analysis range	Measurement range: 10 nm – 3,500 µm Dry Powder System Module (DPS): 400 nm – 2,000 µm Universal Liquid Module (ULM): 10 nm – 2,ooo µm
Electrical interface	USB
Power consumption	≤ 6 amps @ 90 – 125 VAC ≤ 3 amps @ 220 – 240 VAC
Temperature range	10 – 40°C (50 – 104°F)
Humidity	0 – 90% without condensation
Compliance	Creates 21 CFR Part 11 enabling features RoHS Certifications: – EU EMC Directive 2014/30/EU – CISPR 11:2009/A1:2010 – Australia and New Zealand RCM Mark
Data export file formats	XLSX, TSV, PDF
File import capability	From all LS 13 320 Legacy and LS 13 320 XR system
*Software operating system	Requires Microsoft Windows 10, 64-bit environment (US, English regional settings only)
Dimensions	Height: 19.5″ (49.53 cm) Width: 37″ (93.98 cm) Depth: 10″ (25.4 cm)
Weight	52 lbs (23.5 kg)

Accessories
Dry Powder System

Analytical size range: 400 nm – 3,500 µm
- Measures entire sample as required by the ISO 13 320 Standard
- Programmable Obscuration setting to optimize sample feed rate
- User-selectable vacuum pressure for maximum dispersion control
Universal Liquid Module

Analytical size range: 10 nm – 2,000 µm
- Fully automatic with auto-dilution, auto-filling and auto-rinsing
- Analyses samples suspended in aqueous as well as non-aqueous diluents for maximum flexibility
- Wetted materials list: Teflon®, 316 Stainless Steel, Glass, Kal-rez® and PEEK
Sonicator Control Unit
- Needle probe sonicator for additional dispersion control of wet samples
- Fully adjustable power settings
- In-situ sonication with ULM before/during the run, can also be operated external to module
EU Vacuum Cleaner
- Vacuum pressure range fully adjustable
- Integrated vacuum control unit for optimised vacuum/obscuration settings
- Two vacuum systems to choose from
Applications

Soils

Nanocellulose

Pigments

Cement
Capabilities

The LS 13 320 XR particle size analyzer uses advanced laser diffraction and PIDS technology for the sizing of non-spherical, sub-micron particles. Initially, particle sizing by laser diffraction was limited to the use of the Fraunhofer diffraction theory. Laser diffraction offers a number of advantages – laser diffraction analyzers go beyond simple diffraction effects. General approaches are now based on the Mie theory and the measurement of scattering intensity over a wide scattering angular range is employed.

Using PIDS Technology

Pioneered by Beckman Coulter, most laser diffraction manufacturers use the above two approaches, i.e., wide angular detecting range and short wavelength, to size small particles. However, sizing even smaller particles (tens of nanometers in diameter), cannot be achieved using only these two approaches. Any further increase in scattering angle will not yield any significant improvement due to the everslower angular variation. Figure 2 is a 3-D display that illustrates the very slow angular variation for small particles. For particles smaller than 200 nm, even by taking advantage of the above two approaches, it is still difficult to obtain an accurate size.

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Multisizer 4e

Written by Sophie Lindsey on February 7, 2024. Posted in Home page favourites, Products. 1 Comment on Multisizer 4e

Beckman Coulter
Multisizer 4e

Coulter Coulter – Particle Size and Count Analyser

Unparalleled sizing range of 0.2 – 1600 μm
Advanced noise reduction system
Patented digital pulse processing technology

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The Multisizer 4e provides accurate particle and cell counting. It is the most accurate and flexible particle characterisation device available, boasting an unparalleled sizing range of 0.2 – 1600 μm. The new 10 μm Aperture allows users to study sub-cellular and micro-particles as small as 200 nm, while the advanced noise reduction system for small apertures improves measurement accuracy.

Accurate particle and cell counting

The Multisizer 4e Coulter Counter is used in a number of different fields for counting and sizing both particles and cells. Applications include: Quality Control, Research and Development, Pharmaceutical Analysis, Biotechnology Applications and Industrial Applications.

Coulter Principle

Key Features
Versatility
- Aperture sizes from 10 µm – 2000 µm in diameter for current & future needs
- Multiple aperture options & data overlay capability for analyzing complex samples over wide particle size distribution
Sample Detection
- Analyse materials in aqueous or non-aqueous solvents
- Ideal for biological or non-biological samples
- Detect particles regardless of material type or optical properties
Rapid Particle Counting
- Typical sampling rates up to 10,000 counts per second
- Detect & count particles from 0.2 µm – 1600 µm in diameter
- Sample volumes as small as 5 mL
Data Management
- Digital pulse data can be stored & re-analysed as needed
- CFR 21 Part 11 compliant software for audit control & security
- Variety of calibration beads & V-check validation controls

Technical Specs

Particle Sizing Range	Diameter: 0.2 – 1,600 µm Volume: 0.004 – 2.145 x 10⁹ µm³
Aperture Size	10 – 2,000 µm (nominal diameter)
Measurement range	Extended: 2 – 80% of aperture size Standard: 2 – 60% aperture size
Measurement linearity	Diameter: ± 1% Volume: ± 3%
Dynamic range (accuracy)	Diameter: 1 : 40 (extended), 1 :30 (standard), Volume: 1 : 64,000 (extended), 1: 27,000 (standard)
Processor type	High speed signal digitalisation
Number of pulses measured	Up to 525,000 per analysis
Resolution	User difined
Number of size classes	Up to 400 for display of any selected measurement range The number of classes and their width can be changed as needed
Pulse distribution data	X axis: time, registration sequence, pulse width Y axis: diameter, volume or voltage corresopnding to pulse amplitude, pulse width, mean diameter, volume, or voltage corresponding to pulse amplitude, average pulse width, pulse distribution by width
Particle size distribution data	X axis: diameter, volume, surface area Y axis: absolute number, percent content (%), number per ml, absolute volume, volume percentage (%) volume per ml, absolute surface area (%) surface area per ml
Sample registration mode: total number of particles	50 – 500,000 counts
Sample registration mode: number of particles and measurement of parameters	10 – 100,000 counts
Sample registration mode: time	0.1 – 999 seconds with 10 ms increments Standard registration time is 10 – 90 seconds
Sample registration mode: volume	50 – 2,000 µL
Dosage system	The dosing pump with even suspension flow across the aperture and volume measurement, error – less than 0.5%
Electrolyte type:	Aqueous and non-aqueous electrolyte solutions compatible with glass, fluoropolymers, fluoroelastomers and stainless steel
Aperture current strength range:	30 – 6,000 µA with 0.2µA increments
Aperture current stability:	± 0.4% of set value
Polarity error	Less than 0.5%
Compliance with standards	Software is 21 CFR part 11 compliant
Dimensions	64 x 61 x 51 cm, weight 45 kg
Power supply requirements	230 – 240 V ± 10%, 47 – 63 Hz
Power consumption	Less than 55 Watts
Fuses	250 V, IEC (5×20 mm) with time delay, 2.0 A
Environmental requirements	The instrument is intended for work in enclosed spaces Working temperature: 5 – 40°C Relative humidity: 30 – 85% without condensation

Applications

Yeast

Beer

Protein

E. coli

Plant Cells

CAR-T Cells
Capabilities
The Multisizer 4e particle sizer and counter is the most accurate and flexible particle characterization device available, boasting an unparalleled sizing range of 0.2 – 1600 μm. The new 10 μm Aperture allows users to study sub-cellular and micro-particles as small as 200 nm, while the advanced noise reduction system for small apertures improves measurement accuracy.

Generated data are processed using patented digital pulse processing technology and can be saved and later re-analyzed. This technology provides ultra high resolution and accuracy unattainable through any other technologies: detection of 1 particle in 1 ml of a sample with the optimal instrument configuration. Analysis results are not dependent on particle shape, structure, or optical properties.

It uses the Coulter principle to detect particles via electrical zone sensing, regardless of the particle’s nature or optical properties. This makes it an ideal tool for detecting and counting a wide variety of particles, such as:
- Mammalian cells
- Bacteria
- Yeast
- Abrasives
- Toner particles
- Cell aggregates
- Spheroids
- Large protein aggregates
More information from
Beckman Coulter

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Bettersizer S3 Plus

Written by Sophie Lindsey on February 6, 2024. Posted in Home page favourites, Products.

Bettersize
Bettersizer S3 Plus

Particle Size and Shape Analyser

0.01 – 3,500 µm (laser system)
2 – 3,500 µm (image system)
0.5x and 10x cameras
Rapidly generates results in 10 seconds

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Strive for excellence in all you see.

The Bettersizer S3 Plus particle size and shape analyser combines laser diffraction and dynamic image analysis in one instrument. It can measure the size and shape of particles from 0.01 µm to 3500 µm. Its exceptional sensitivity for either ultrafine particles or oversized particles, and unsurpassed resolution, make it the most powerful size and shape analyser for enthusiastic researchers who conduct top scientific research.

Features and Benefits
- Measuring range is 0.01 – 3,500µm (laser system), 2 – 3,500µm (image system)
- Combining laser diffraction and dynamic image analysis in one instrument, obtaining size and shape results simultaneously
- Patented DLOI (Dual Lenses & Oblique Incidence) system enable the measurement of ultrafine particles down to 0.01 µm
- Dual-camera imaging technology can show particles images in real time and detect oversized particles up to 3500 µm
- Refractive index measurement determines the refractive index of unknown samples and improves te reliability of results
- Compliance with 21 CFR Part 11, ISO 13320, USP <429>, CE
Performance

1) High Resolution and Sensitivity

The Bettersizer S3 Plus achieves exceptional resolution and sensitivity for particle size measurements. The DLOI system allows the size distributions of polydisperse samples to be determined precisely, and the size changes of products to be detected sensitively.

2) Multiple Shape Parameters

An example of additive manufacturing for shape analysis using the Bettersizer S3 Plus is shown below. A representative number of individual particles are recorded from two AlSi10Mg samples, and the number-weighted aspect ratio and circularity are evaluated in compliance with ISO standards. (Adapted from F. Schleife, C. Oetzel. Chem. Ing. Tech. 93.8 (2021): 1199–1203.)

3) Oversized Particle Detection

Laser diffraction in combination with image analysis can sensitively detect oversized particles that are statistically underrepresented within a wide-distributed sample, such as oversized grains, agglomerates, air bubbles, etc. An example of an off-specification abrasive is displayed below. The Bettersizer S3 Plus confirms the presence of oversized particles, by showing a size peak at around 120 μm and the images of overly coarse particles.

4) Analysis of Samples with Extremely Broad Distributions
Technology

Image Analysis

Laser Diffraction
Applications

Soy Milk

Advanced Ceramic Products

Powder Coatings

Ceramic Agglomerates

Lithium-Ion Batteries

Soils and Sediments

Abrasives

3D Printing

Differing Abrasives

Specification

Particle size distribution	Suspension, emulsion, dry powders
Particle shape	Suspension, emulsion, dry powders
Principle	Laser diffraction and dynamic image technologies
Analysis	Mie scattering theory and Fraunhofer diffraction theory
Typical measurement time	Less than 10 seconds
Measuring range	0.01 – 3500 μm (Laser System) 2 – 3500 μm (Image System)
Accuracy	<0.5% (NIST certified standards)
Repeatability	<0.5% (NIST certified standards)
Number of size classes	≤100 (adjustable)
Feeding mode	Automatic circulation or semi-automatic circulation
Special functions	Refractive index measurement, SOP settings
Image recognition	Up to 120 fps, up to 10,000 particles per min
Optical system	Patented DLOI (Dual Lenses & Oblique Incidence) System
Laser	Polarized light-pumped solid-state laser (10 mW / 532 nm)
Detector	96 detectors (forward, lateral and backward arrangements)
Measuring angle	0.02 – 165°
CDC cameras	0.5x and 10x *
Image analysis	1.2 megapixels
Circulation speed	300 – 2500 r/min
Circulation flow rate	3000 – 8000 mL/min
Ultrasonication	Dry run protection, Max 50 W (adjustable)
Circulation tank capacity	600 mL
Conformity	21 CFR Part 11, ISO 13320, ISO 13322, USP <429>, CE
Report	Customizable reporting
Dimensions (L x W x H)	820 × 610 × 290 mm
Weight	48 kg
Voltage	DC 24 V, 50 / 60 Hz, 20 W
Computer interface	At least one high-speed USB 2.0 or USB 3.0 port required
Operating system	Windows 7 / Windows 10
Hardware specification	Intel Core i7, 8GB RAM, 500GB HD, two PCI-E X16 interfaces

* The Bettersizer S3 Plus is also available in a single camera (0.5x) model. Contact us for more information.

Accessories
Introduction

The BT-A60 is a durable, automatic and high-throughput sampling system. It delivers maximum laboratory automation for sample measurements, reducing your labor costs while improving productivity and laboratory efficiency. The compact design saves valuable bench space while allowing up to 60 different samples to be measured in a single run. Compatible with Bettersizer S3 Plus and Bettersizer 2600, the BT-A60 offers 24/7 fully automated sample analysis to meet your various analytical applications.

Features
- Accurate sample identification
- Efficient ultrasonic cleaning
- Up to 60 samples in one click
- Measurement automation
- Small footprint
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Bettersizer 2600 Plus

Written by Sophie Lindsey on February 5, 2024. Posted in Products.

Bettersize
Bettersizer 2600 Plus

Laser Diffraction and Dynamic Image Analysis

Size and Shape in One Platform: a combination of laser diffraction and dynamic imaging
Wide Range: 0.02–3500 μm for most materials and workflows
Modular Dispersion Units: flexible wet and dry setups for different samples and solvents

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$Bettersizer 2600 Plus ● Size and Shape in One Platform: a combination of laser diffraction and dynamic imaging ● Wide Range: 0.02–3500 μm for most materials and workflows ● Modular Dispersion Units: flexible wet & dry setups for different samples and solvents$

Bettersizer 2600 Plus combines laser diffraction and dual-camera dynamic imaging on one modular platform, delivering size and shape characterization across 0.02–3500 μm. Laser diffraction provides fast, repeatable particle size distributions using a 92-detector array and Mie/Fraunhofer models. Dynamic imaging captures high-speed particle images to quantify size and shape, with visual evidence for agglomerates, irregular particles, and oversized tails. With a modular design, Bettersizer 2600 Plus supports a broad selection of wet and dry dispersion units. This enables flexible setups for different sample types, volumes, and solvents, so one instrument can meet diverse applications and industries

Key Features

Principle	Laser Diffraction technology, Dynamic Image Analysis
Analysis	Mie scattering theory, Fraunhofer diffraction theory, Dynamic image analysis
Data acquisition rate	11 kHz
Typical measurement time	Less than 10 seconds
Measurement range	0.02–2,600 μm (wet); 0.1 – 2,600 μm (dry); 2-3,500 μm (dynamic image)
Acuracy	≤ 0.5%
Repeatability	≤ 0.5%
Number of size classes	100 (adjustable)
Optical System	Laser Diffraction System
Laser	10 mW, 635 nm
Detector	92 detectors
Measuring angle	0.016-165°
Alignment	Automatic
Optical System	Dynamic imaging system
CMOS Camera	0.5x and 10x
Measuring range	2-3,500 μm
Frame rate	70 fps at 5 MP
Laser	Class 1 (IEC60825-1 and 21CFR1040.10)
Regulatory	RoHS, CE
Standards	ISO 13320, ISO 13322-2, USP <429>
Software	21 CFR Part 11
Supply voltage	100-240 VAC, 50/60 Hz
Dimensions (LxWxH)	745 × 305 × 305 mm
Weight	33 kg
Computer interface	At least two high-speed USB 2.0 and two USB 3.0 port required
Operating system	Windows 10 or higher
Hardware specification (recommended)	Intel Core i5 Processor, 16 GB RAM, 512 GB SSD, 1920 × 1080 (Full HD)

Dispersion Units

Bettersizer 2600 Plus Family

Wet Dispersion Units

Consistent Dispersion Workflow for Reliable Results

Wet dispersion introduces the sample into a selected liquid medium, such as water or a compatible organic solvent, to form a stable suspension. A controlled workflow, typically defined by automated SOPs, keeps dispersion conditions consistent.

Continuous circulation and stirring maintain homogeneity, integrated ultrasonic breaks agglomerates and helps release entrapped air, and the suspension passes through the optical measurement zone and is recirculated for repeatable analysis. Automated surfactant addition further improves wetting and suspension stability, reducing re-agglomeration and supporting reliable, operator-independent results.

Core Functions Across Wet Dispersion Units

Stirring and circulation:	Ultrasonic energy:	Surfactant addition:
maintain uniformity and reduce settling	deagglomerate particles and help remove trapped air	improve wetting and stabilize the suspension

BT-812 Automatic Wet Dispersion Unit	Most routine samples can be dispersed in water, so water-based wet dispersion is the most common workflow. For these applications, BT-812 is the primary wet dispersion unit for Bettersizer 2600 Plus, combining controlled circulation, ultrasonics, and automated liquid handling to deliver repeatable measurements.
BT-812 Automatic Wet Dispersion Unit	• High-capacity circulation tank, maximum volume is 500 mL • Centrifugal circulation pump with adjustable speed • 50 W adjustable ultrasonic dispersion with dry-run protection • Fully automated liquid handling system, including level monitoring and automatic dispersant addition • Full software control for dispersion, measurement, and cleaning workflows
	Parameter	Specification
	Measurement range*	0.02 – 2,600 μm
	Stirring speed	300 – 2,500 rpm
	Ultrasonic power	50 W max
	Volume	500 mL max
	Medium	Water
	SOP	Yes
	Dimensions (LxWxH)	257 x 275 x 308 mm (LxWxH)
	Weight	11.5 kg
	Component	ABS housing 304 stainless steel tank Silicone tubing Centrifugal pump Peristaltic pump Ultrasonic disperser Quartz sample cell Pinch valve
	* Sample and sample preparation dependent


Automatic Surfactant Addition	Easy-to-Remove Wet Sample Cell
Automatic surfactant dosing during dispersion Volume-controlled addition for consistent preparation SOP settings for standardized workflows	Toggle-lock mechanism for quick installation and removal Tool-free removal for everyday convenience

BT-80N Pro Automatic Anti-Corrosive Wet Dispersion Unit	BT-80N Pro is designed for wet dispersion workflows that require organic solvents or corrosive media.To meet these applications, key components are built with corrosion-resistant materials, improving long-term compatibility and reliability.
	Low-volume circulation tank: 80–120 mL Broad organic solvent compatibility Centrifugal circulation pump with adjustable speed 50 W adjustable ultrasonic dispersion with dry-run protection Full software control for dispersion, measurement, and cleaning workflows Dual operation modes: local panel control or software control
	Parameter	Specification
	Measurement range*	0.02 – 2,600 μm
	Stirring speed	300 – 2,500 rpm
	Ultrasonic power	50 W max
	Volume	80 – 200 mL
	Medium**	Water, organic solvent
	SOP	Yes
	Dimensions (LxWxH)	240 × 220 × 290 mm (LxWxH)
	Weight	9 kg
	Key Component	316L Stainless-steel housing PTFE tubing 316 Stainless-steel diaphragm pump 316L Stainless-steel tank Quartz sample cell Ultrasonic disperser
	* Sample and sample preparation dependent ** Compatibility depends on the wetted materials. Please contact Bettersize to confirm compatibility with your solvent.

BT-80N Anti-Corrosive Wet Dispersion Unit	The BT-80N is a cost-effective, entry-level solution that provides essential solvent compatibility for routine measurements.
BT-80N Anti-Corrosive Wet Dispersion Unit	Compact circulation tank: 50–80 mL Centrifugal circulation pump with adjustable flow 50 W adjustable ultrasonic dispersion with dry-run protection Broad organic solvent compatibility Operation modes local panel control
	Parameter	Specification
	Measurement range	0.02 – 2,600 μm
	Stirring speed	300 – 3,000 rpm
	Ultrasonic power	50 W max
	Volume	50 – 80 mL
	Medium	Water, organic solvent
	SOP	No
	Dimensions (LxWxH)	290 × 210 × 375 mm (LxWxH)
	Weight	11 kg
	Key Component	316L Stainless-steel housing PTFE tubing 316L Stainless-steel tank Quartz sample cell Ultrasonic disperser

BT-814 Small-Volume Wet Dispersion Unit	The BT-814 is specifically designed for measurements where sample availability is limited or material value is high. It supports dispersion in both water-based and organic solvents, providing maximum flexibility across a wide range of applications.
BT-814 Small-Volume Wet Dispersion Unit	Small-volume sample cell: 8 mL Broad dispersion media compatibility Controlled stirring for stable dispersion conditions
	Parameter	Specification
	Measurement range*	0.02 – 2,600 μm
	Volume	8 mL
	Medium	Water or organic solvent
	SOP	No
	Dimensions	/
	Weight	/
	Key component	ABS housing Quartz sample cell 316L Stainless-steel stirrer
	* Sample and sample preparation dependent

Dry Dispersion Units

Controlled Deagglomeration for Repeatable Results

Dry dispersion introduces powder samples into the system with gases as media. The sample is delivered into the disperser by controlled vibration. Inside the disperser, particles are accelerated by a precisely controlled high-pressure gas stream through a Venturi device. Shear forces, interparticle collisions, and particle–wall collisions break down agglomerates before measurement. The dispersed particle stream then passes through the laser diffraction measurement zone for analysis, and is safely evacuated and collected by a vacuum unit.
Shear forces high-velocity airflow separates loosely bound agglomerates during acceleration and transport
Inter-particle collisions: collisions under controlled flow promote deagglomeration and dispersion stability
Particle–wall impacts: impacts with the inlet pathway enhance agglomerate breakup before measurement

BT-912 Automatic Dry Dispersion Unit
The BT-912 is the primary dry dispersion unit for routine powder dispersion using compressed gas. It supports a wide range of powders, including friable and cohesive powders.	Parameter	Specification
	Measurement range*	0.1 – 2,600 μm
	Powder mass	0.2 – 10 g
	Gas pressure	0.1 – 0.8 MPa
Representative sampling: 0.2–10 g for standard laboratory analysis and QC Controlled feeding: adjustable hopper position and vibration frequency to stabilize obscuration Flexible gas supply options: compatible dispersion for diverse dry powders Fully automated sample feeding and cleaning: improves efficiency and consistency	Funnel height	0.7 – 2.9 mm
	Medium	Air, nitrogen or noble gases
	SOP	Yes
	Dimensions	276 x 189 x 243 mm
	Weight	8 kg
	Key Component	304 Stainless-steel funnel 304 stainless-steel feeder Venturi disperser Vacuum cleaner (optional) Air Compressor (optional)
	* Sample and sample preparation dependent

BT-903 Small-Volume Dry Dispersion Unit
BT-903 is the small-volume dry dispersion unit, designed for powder measurements when sample amount is limited. Samples are preloaded in a tube and introduced into the disperser by controlled gas flow and negative-pressure suction, enabling stable and repeatable sampling.	Parameter	Specification
	Measurement range*	0.1 – 2,600 μm
	Powder mass	0.02 – 1 g
Negative-pressure sampling: stable small-quantity feeding with 0.02 – 1 g sample dosage Quick tube replacement: reduces carryover Removable glass tube: easier maintenance and thorough cleaning Fully automated feeding and cleaning: improves efficiency and consistency Flexible gas supply options: compatible dispersion for diverse dry powders	Tube volume	5 mL max
	Gas pressure	0.1 – 0.8 MPa
	Medium	Air, nitrogen or noble gases
	SOP	Yes
	Dimensions	195 × 260 × 245 mm
	Weight	5 kg
	Key Component	Plastic sample tube 316 Stainless-steel tubing Silicon tubing Venturi disperser Vacuum cleaner (optional) * Sample and sample preparation dependent Air Compressor (optional)

Module Selection

Easy Module Selection

The Bettersizer 2600 Plus features both wet and dry dispersion units for effective particle dispersion, along with a dynamic imaging module to expand the measurement range and perform particle shape analysis. Our decision tree* for module selection helps users choose the right dispersion unit and determine if the dynamic imaging module is necessary.
Applications

Pigments

Coffee

Chocolate

Ceramic Powders

Lithium Batteries

Powder Coatings

Abrasives

Mineral Pigment

Pharmaceuticals

Powdered Milk

Cement

Coffee 2

Lactose

Pharmaceuticals 2

Chinese Medicine
Technology

Laser Diffraction

Software

Smart and Powerful Software

Bettersizer Software is designed to optimize the entire particle size measurement workflow, covering every stage from sample preparation and measurement control to data analysis and report generation. Seamlessly integrated with the Bettersizer 2600 Plus, it streamlines routine operations through a high level of automation, reducing manual intervention while improving efficiency and result consistency.

Real-time laser diffraction view: monitors obscuration and signal status while updating the particle size distribution during the run.
Dynamic imaging window: supports image-based testing to add particle shape information alongside size results.
Individual particle view: provides image-level review of captured particles
Imaging size results: displays image-derived particle size distributions for verification and extended coverage.
Shape scatter plots: visualizes size–shape relationships to distinguish particle distribution and shape trends.
Test record table: organizes run history and key parameters for traceability, comparison, and reporting.

Smart Dispersion Control

SOP-Based Automation

Flexible Measurement Modes

“Auto Dispersion Setting” automatically determines the optimal dispersion time based on real-time repeatability. It stops when the user-defined repeatability target is met, or selects the best-repeatability interval at the maximum time.

SOPs standardize the full workflow beyond basic stirring, ultrasonics, and circulation. Automated functions can include auto dilution to maintain target obscuration, automatic dispersant addition, and report generation/printing, improving consistency across operators and laboratories.

Users can select laser diffraction, dynamic imaging, or combined LD and imaging modes to match sample characteristics and reporting needs, enabling both particle size and shape characterization within a unified workflow.

Software Features Across the Testing Workflow

1. Automatic Pre-processing	2. Automated System Preparation	3. Real-time Testing	4. Data Evaluation and Reporting
Users can easily create new tests based on laser diffraction and dynamic image analysis methods. The software supports both automated and manual control, providing flexibility for various sample types and testing conditions. The SOP offers a streamlined solution for standardized and automatic testing, ensuring operator-independent results that are consistent and reliable.	The Bettersizer software significantly enhances data quality by automating critical instrument functions like system cleaning, optical alignment, and sample dispersion. These automated processes ensure optimal instrument performance, leading to increased precision, accuracy, and reproducibility of results.	During the testing process, Bettersizer software delivers real-time insights into particle size distribution and shape. These immediate results provide valuable information on test progress and outcomes, enabling precise adjustments to achieve optimal results.	The Bettersizer software excels in delivering comprehensive data analysis and report generation capabilities. The software allows users to customize and edit reports to meet specific requirements, including various data points, charts, and graphical representations, to create clear and informative reports. The data evaluation tools can help in assessing the result quality.
Combined Test	Automatic Dispersion Settings	Imaging Analysis Window	Data Interface
New SOP Screen	Auto-alignment	Laser Diffraction Analysis Window	Data Evaluation

Reporting and Data Export

Highly Customizable Report

● Complete and detailed data: Frequency and cumulative distribution curves, simplified and complete distribution table, etc.
● Editability: Users can easily edit the reports and change the font, layout, format, etc.
● Convertibility: Users can switch the formats of reports freely among PDF, Excel, Text, etc.

Citations

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Bettersizer ST

Written by Sophie Lindsey on February 4, 2024. Posted in Products.

Bettersize
Bettersizer ST

Particle Size Analyser – Wet Analysis

Size range 0.1 – 1,000 µm
Low maintenance
Cost-Efficiency

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$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

The Bettersizer ST is a fully automated and integrated particle size analyser with a smart operation system by wet dispersing. Optimised for the industrial quality control process, the Bettersizer ST provides stable and reliable testing results with minimum user intervention. The compact footprint saves valuable workspace for factories and laboratories.

Particle Size Analyser you can TRUST

The Bettersize Bettersizer ST Particle Size Analyser ensures reliable wet analysis for quality control processes. Utilising laser diffraction, it provides accurate and reproducible measurements. Ideal for various industries, the Bettersizer ST guarantees precise particle size distribution, enhancing product consistency and quality assurance

Key Features
- Dispersion type: Wet
- Size range: 0.1 – 1,000µm
- Robustness
- Ease-of-Use
- Cost-Efficiency
- Compact design
- Low maintenance
- Dual lens system
- Automatic alignment functionality
- Automatic measurement with SOP

Technical Specs

Particle Size Distribution	Suspensions, emulsions, dry powders
LPrinciple	Laser diffraction technology
Analysis	Mie scattering theory and Fraunhofer diffraction theory
Typical measurement time	Less than 10 seconds
Measurement range	0.1 µm – 1000 µm
Accuracy error	≤1% (NIST certified standards)
Repeatability error	≤1% (NIST certified standards)
Number of size classes	≤100 (adjustable)
FOptical system	Patented DLOS (Dual Lens Optical Systems)
Laser	High-power fiber semiconductor laser (10 mW/635 nm)
Detector	86 photodetectors (forward, lateral and backward arrangements)F
Measuring angle	0.031 – 159°
Circulation speed	300 – 2500 r/min
Circulation flow rate	3,000 – 8,000 mL/min
Ultrasonication	Dry run protection, Max 50 W (adjustable)
Circulation tank capacity	600 mL
Conformity	21 CFR Part 11, ISO 13320, CE
Reports	Customizable reporting
Dimensions (L x W x H)	660 x 420 x 320 mm
Weight	38 KG
Voltage	DC 24V, 221 W
Computer interface	At least one high-speed USB 2.0 or USB 3.0 port required
Operating system	Windows 7 or higher
Hardware specification	Intel Core I5, 4GB RAM, 250GB HD

Applications

Lithium-ion Battery

Calcium Carbonate

Pesticides

Ceramic Powder

Abrasives

Gypsum

Pesticides 2
Technology

Laser Diffraction

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BeNano Series

Written by Sophie Lindsey on February 3, 2024. Posted in Products.

Bettersize
BeNano Series

Nanoparticle Size and Zeta Potential Analyser

Size range: 0.3nm – 15μm
PALS (Phase Analysis Light Scattering) technologyy
Compliance with 21 CFR Part 11, ISO 22412, ISO 13099

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$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

The BeNano Series is the latest generation of nanoparticle size and zeta potential analysers designed by Bettersize Instruments. Dynamic light scattering (DLS), electrophoretic light scattering (ELS), and static light scattering (SLS) are integrated into the system to provide accurate measurements of particle size, zeta potential, and molecular weight. The BeNano Series is widely applied in academic and manufacturing processes of various fields including but not limited to: chemical engineering, pharmaceuticals, food and beverage, inks and pigments, and life science, etc.

Features and Benefits

Size range: 0.3nm – 15μm
Minimum sample volume 3μL
APD (Avalanche Photodiode) detector providing exceptional sensitivity
Automatic adjustment of laser intensity
Intelligent algorithm of result evaluation
DLS backscattering (173°) detection technology
User-adjustable scattering volume for concentrated samples
PALS (Phase Analysis Light Scattering) technology
Programmable temperature control system
Compliance with 21 CFR Part 11, ISO 22412, ISO 13099

Features

The BeNano series comprises seven models and represents a state-of-the-art generation of nanoparticle analysers that integrate light scattering and transmission techniques.

Compare BeNano series models

Features	BeNano 180 Zeta Max	BeNano 180 Zeta Pro	BeNano 180 Zeta	BeNano 90 Zeta	BeNano Zeta	BeNano 180	BeNano 90
Particle Size – 90° DLS	√	√	×	√	×	×	√
Particle Size – 173° DLS	√	√	√	×	×	√	×
Zeta Potential	√	√	√	√	√	×	×
Molecular Weight	√	√	√	√	×	√	√
Microrheology	√	√	√	√	×	√	√
Refractive Index	√	×	√★	√★	×	√★	√★
Concentration	√	×	√★	√★	×	√★	√★
Sedimentation	√	×	√★	√★	×	√★	√★
Transmittance	√	×	√★	√★	×	√★	√★
Temperature Trend	√	√	√	√	√	√	√
VV Polarizer	√★	√★	√★	√★	×	√★	√★
VH Polarizer	√★	√★	×	√★	×	×	√★
Fluorescence Filter	√★	√★	√★	√★	×	√★	√★
Flow Mode	√★	√★	√★	√★	×	√★	√★
Autotitration	√★	√★	√★	√★	√★	×	×
★ Optional

If you’re not sure which model is right for you, feel free to contact us here.

1. Particle Size Measurement — Dynamic Light Scattering (DLS)

Dynamic Light Scattering (DLS), also known as Photon Correlation Spectroscopy (PCS) or Quasi-Elastic Light Scattering (QELS), is a technique used to determine particle size by analysing the Brownian motion of particles in a dispersion. DLS is based on the principle of Brownian motion, which relates particle size to velocity—smaller particles diffuse more rapidly, while larger particles move more slowly. The scattering intensities of the particles are detected by an avalanche photodiode (APD) and then converted into a correlation function. From this correlation function, a mathematical algorithm can be applied to obtain the diffusion coefficient (D). The hydrodynamic diameter (D_H) and its distribution can be calculated using the Stokes-Einstein equation, which relates the diffusion coefficient to the particle size.

1.1 Backscattering Detection Technology

Using backscattering optics, the analyser automatically identifies the best detection position by evaluating the sample’s size, concentration, and scattering characteristics. This ensures maximum measurement accuracy while offering the adaptability needed to evaluate a wide range of samples with varying properties.

Features

- Wider Concentration Range: By optimising the detection position, highly concentrated samples can be detected near the edge of the sample cell, effectively minimising errors from multiple light scattering.

- Increased Sensitivity: Offers 8-10 times the scattering volume and approximately 10 times greater sensitivity as compared to traditional 90° optics.

- Expanded Size Detection Range: By minimizing multiple light scattering from larger particles, this approach enhances measurement accuracy. Additionally, the significantly larger scattering volume helps reduce the number fluctuations of large particles, leading to more reliable analysis.

- Better Reproducibility: Reduced effects from dust contaminants and unevenly distributed agglomerates, improving reproducibility

2. Zeta Potential Measurement — Electrophoretic Light Scattering (ELS)

In aqueous systems, charged particles are surrounded by counter-ions that form an inner Stern layer and an outer shear layer. Zeta potential is the electrical potential at the interface of the shear layer. A higher zeta potential indicates greater stability and less aggregation of the suspension system. Electrophoretic light scattering (ELS) measures electrophoretic mobility via Doppler shifts of scattered light, which can be used to determine the zeta potential of a sample by Henry’s equation.

Colloidal Stability

Stable particle system	Unstable particle system

High repulsion force of particles High zeta potential	Flocculation, aggregation, sedimentation Low or zero zeta potential

2.1 Phase Analysis Light Scattering (PALS)

PALS is a more advanced technique than traditional ELS, which has been further developed by Bettersize to measure the zeta potential.

Features and Benefits

- Accurate measurement of samples with low electrophoretic mobility
- Effective for samples in organic solvents with low dielectric constant
- More accurate results for samples with high conductivity
- Effectively measures the zeta potential of particles whose charge approaches the isoelectric point

3. Molecular Weight Measurement — Static light scattering (SLS)

Static light scattering (SLS) is a technique that measures scattering intensities to calculate the weight-average molecular weight (Mw) and the second virial coefficient (A2) of a sample using the Rayleigh equation.

where c is the sample concentration, θ is the detection angle, R_θ is the Rayleigh ratio used to characterise the intensity ratio between the scattered light and the incident light at the angle of θ, M_w is the sample’s weight-average molecular weight, A₂ is the second virial coefficient, and K is a constant related to (dn/dc)².

Features & Benefits

Non-invasive technique
Suitable for particles dissolved in liquid
Measures molecular weight of samples smaller than 30 nm
Provides second virial coefficient A₂, indicating the intermolecular interactions

Debye Plot

4. DLS Microrheology Measurement

Dynamic Light Scattering Microrheology (DLS Microrheology) is an economical and efficient technique that utilizes dynamic light scattering to determine rheological properties. By analyzing the Brownian motion of colloidal tracer particles, information about the viscoelastic properties of the system, such as viscoelastic modulus, complex viscosity and creep compliance, can be obtained with the generalized Stokes-Einstein equation.

Features & Benefits

Investigates rheological behaviors by measuring the thermally-driven motion of tracer particles within a material being studied
Facilitates the measurement of a broad frequency range in a single measurement
Suitable for dilute, weakly structured solutions
Delivers fast results in 1–2 minutes with easy operation
Offers rheological insights across a wide temperature range from -15°C to 120°C
Complements conventional mechanical rheology

5. DLS Flow Mode Measurement

DLS flow mode provides a high-resolution size result of a complex, polydisperse system. When combined with front-end separation equipment such as GPC/SEC or FFF, particles are separated into monodisperse fractions and flow through the BeNano in sequence by size. The size of each fraction is continuously measured and summed into a high-resolution size distribution.

BeNano can acquire RI or UV signals, offering a more accurate volume and number distributions independent of the algorithm compared to a batch-mode measurement.

Features & Benefits

DLS analyser connecting with GPC/SEC, FFF, etc.
Receiving up to 3 signals from RI, UV, or other detectors
27 μL low-volume flow cell to avoid band broadening
Size resolution as high as 1.3:1
Size distributions weighted by number and volume, in addition to intensity
Suitable for complex, polydisperse systems such as proteins, polymers, etc.

6. Temperature Trend Measurement

Features & Benefits

Programmed temperature trend measurement from -15°C to 120°C
Important for analysing particle size and zeta potential across varying temperatures
Easy examination of protein formulation stability
Accelerates real-time aging through elevated temperature simulation

7. Transmittance Measurement

Features & Benefits

Measures transmittance rapidly by detecting the light intensity transmitted through the sample
Requires a minimum sample volume of 3 μL
Sensitive indicator for evaluating batch consistency in industrial products
Quantitative tool for identifying sample instability

8. Refractive Index Measurement

The BeNano Series can determine the refractive index (RI) measurement of liquids with outstanding precision. A patented wedge-shaped cuvette holds the liquid sample while the CMOS detector measures the deflection of the light path after it traverses the liquid to calculate the RI.

Features & Benefits

Patented technique supports a broad refractive index range from 1.2 to 1.6
Requires only two calibration references and utilizes linear calibration suitable for extrapolation
No tracer particles or prior knowledge of viscosity are required
Enables DLS and ELS measurement for dispersants with unknown refractive indices
Suitable for both organic and aqueous solvents

9. Concentration Measurement

The BeNano measures particle volume fraction and number concentrations in particles per milliliter (particles/mL) for each population through the patented LEDLS technique. The incident light passes through the sample and reaches a photodiode detector, which records the transmitted intensity. By comparing it with that of a blank solution and combining the data with the particle size distribution from dynamic light scattering, the particle concentration is determined.

Features & Benefits

Enables fast measurement with single-angle Detection
Simplifies sample preparation with no need for calibration
Ideal for screening-type measurements
Suitable for both aqueous and organic samples

10. Sedimentation Size Measurement

The BeNano Series provides particle size results based on the sedimentation method. The sedimentation rate of particles is directly related to their size, with larger particles settling faster. The PD detector monitors the changes in transmitted intensity over time, enabling the determination of particle size and distribution for particles up to 50 microns.

Schematic of the sedimentation method

Features & Benefits

Expands size measurement range up to 50 μm
Suitable for samples containing both nanoparticles and microparticles, meeting the needs of broad distribution samples
Provides volume-based size distributions for micron-sized particles, consistent with laser diffraction results
Achieves up to 1.5x size resolution for multiple peaks

11. pH Autotitration Measurement

The BAT-1 + Degasser units integrate seamlessly with the BeNano Series for automatic acid-base titration and isoelectric point (IEP) determination. The system automatically enables sample flow during measurement, ensuring high efficiency and consistent, operator-independent results, as well as precise titration.

An optional degasser is available to remove dissolved gases from titrants. Preventing bubbles improves the accuracy of zeta potential measurements.

Features & Benefits

Accurate size and zeta potential analysis from pH 1 to 13
Enhanced safety with minimal exposure to corrosive liquids
Automated workflow reduces training needs and researcher workload
Fewer manual steps minimise human error
Completes each measurement cycle in as little as 30 minutes
Smart Titration: Based on the initial pH and the target pH, the required titrants can be chosen automatically via the software

Technology
Accessories
BAT-1 Autotitrator

1) Introduction

The BAT-1 Autotitrator is equipped with three high-precision titration pumps (with precision of 0.28 μL), and a magnetic stirrer, and is in combination with the BeNano series nanoparticle size and zeta potential analyzer for automatic acid-base titration and determination of isoelectric point (IEP). The pinch valve can close the circuit of the sample during the measurement, leading to high efficiency, accurate titration, good repeatability and the results being independent of operators. The disposable sample container can avoid the sample cross-contamination.

2) Features
- Combination electrode with high precision and high feedback speed
- High precision ternary titration pumps
- Controllable peristaltic pump with high flow capacity and high flow rate
- Internal magnetic stirrer system
- SOP operation
- Replaceable tubes
- Corrosion resistant design
- General purpose electrode
- Intelligentization
- Determination of isoelectric point
3) How it works

The BAT-1 Autotitrator is designed to be used with the BeNano series for the measurement of zeta potential over a wide pH range, providing the information of zeta potentials and the stability of samples in different conditions. The operation flow is as follows:
- a). Preparing the samples to be detected and the titrants in the containers, respectively;
- b). Creating or editing a titration SOP in BeNano software by setting the volume of the sample to be measured, the concentrations of the titrants, the initial pH, the target pH, the pH interval and the target pH tolerance, etc.;
- c). To start the determination, the sample is titrated to approach to the first pH value through automatic calculation, and is injected into the folded capillary cell by the peristaltic pump for zeta potential measurement;
- d). Repeating the above procedures until approaching the final target pH automatically;
- Saving and outputting complete data and the trend plot of zeta potential vs. pH;
- Giving the isoelectric point if it is included in the setting pH range.
4) Download BAT-1 Autotitrator Flyer
Applications

Battery Electrode Slurry

Monosaccharide Molecules

Resins

BSA Solution

Aluminium Oxide

Titanium Oxide

Thermal Sensitive Hydrogel

Latex antibody immunological reagents

Alumina Abrasives

Iron Dextran

Fat Emulsion

BSA Solution

Surfactant Micelles

Pigment

Bovine Serum Albumin

Coffee Creamer Powder

Lysozyme

Specification

Functions	Parameter	BeNano 180 Zeta Pro	BeNano 180 Zeta	BeNano 90 Zeta	BeNano Zeta	BeNano 180 Pro	BeNano 180	BeNano 90
Size measurement	Size measurement range	0.3 nm – 15 μm*	0.3 nm – 10 μm*	0.3 nm – 15 μm*	N/A	0.3 nm – 15 μm*	0.3 nm -10 μm*	0.3 nm – 15 μm*
	Sample volume	3 μL – 1 mL*	40 μL – 1 mL*	3 μL – 1 mL*	N/A	3 μL – 1 mL*	40 μL – 1 mL*	3 μL – 1 mL*
	Detection angle	90° & 173° & 12°	173° & 12°	90° & 12°	N/A	90° & 173°	173°	90°
	Analysis algorithm	Cumulants, General Mode, CONTIN	Cumulants, General Mode, CONTIN	Cumulants, General Mode, CONTIN	N/A	Cumulants, General Mode, CONTIN	Cumulants, General Mode, CONTIN	Cumulants, General Mode, CONTIN
	Upper limit of concentration range	40% w/v*	40% w/v*	Optically clear⁺	N/A	40% w/v*	40% w/v*	Optically clear†
	Detection position	Movable position 0.4 – 5 mm	Movable position 0.4 – 5 mm	Fixed position 5 mm	N/A	Movable position 0.4 – 5 mm	Movable position 0.4 – 5 mm	Fixed position 5 mm
Zeta potential measurement	Detection angle	12°	12°	12°	12°	N/A	N/A	N/A
	Zeta potential measurement range	No actual limitation	No actual limitation	No actual limitation	No actual limitation	N/A	N/A	N/A
	Electrophoretic mobility	> ± 20 μm·cm/V·s	> ± 20 μm·cm/V·s	> ± 20 μm·cm/V·s	> ± 20 μm·cm/V·s	N/A	N/A	N/A
	Conductivity	0 – 260 mS/cm	0 – 260 mS/cm	0 – 260 mS/cm	0 – 260 mS/cm	N/A	N/A	N/A
	Sample volume	0.75 – 1 mL	0.75 – 1 mL	0.75 – 1 mL	0.75 – 1 mL	N/A	N/A	N/A
	Sample size	2 nm – 110 μm	2 nm – 110 μm	2 nm – 110 μm	2 nm – 110 μm	N/A	N/A	N/A
Other measurements	Molecular weight (Mw)	342 Da – 2 x 10⁷ Da*	342 Da – 2 x 10⁷ Da*	342 Da – 2 x 10⁷ Da*	N/A	342 Da – 2 x 10⁷ Da*	342 Da – 2 x 10⁷ Da*	342 Da – 2 x 10⁷ Da*
	Viscosity	0.01 cp – 100 cp*	0.01 cp – 100 cp*	0.01 cp – 100 cp*	N/A	0.01 cp – 100 cp*	0.01 cp – 100 cp*	0.01 cp – 100 cp*
	Interaction parameter K_D	No actual limitation	No actual limitation	No actual limitation	N/A	No actual limitation	No actual limitation	No actual limitation
	Trend measurement	Time and temperature	Time and temperature	Time and temperature	Time and temperature	Time and temperature	Time and temperature	Time and temperature
System parameters	Temperature control range	-15℃ – 110℃, ±0.1℃	-15℃ – 110℃, ±0.1℃	-15℃ – 110℃, ±0.1℃	-15℃ – 110℃, ±0.1℃	-15℃ – 110℃, ±0.1℃	-15℃ – 110℃, ±0.1℃	-15℃ – 110℃, ±0.1℃
	Condensation control	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen	Dry air or nitrogen
	Laser source	50 mW Solid-state laser, 671 nm^#, Class 1	50 mW Solid-state laser, 671 nm^#, Class 1	50 mW Solid-state laser, 671 nm^#, Class 1	50 mW Solid-state laser, 671 nm^#, Class 1	50 mW Solid-state laser, 671 nm^#, Class 1	50 mW Solid-state laser, 671 nm^#, Class 1	50 mW Solid-state laser, 671 nm^#, Class 1
	Correlator	Up to 4000 channels, 10¹¹ linear dynamic range	Up to 4000 channels, 10¹¹ linear dynamic range	Up to 4000 channels, 10¹¹ linear dynamic range	Up to 4000 channels, 10¹¹ linear dynamic range	Up to 4000 channels, 10¹¹ linear dynamic range	Up to 4000 channels, 10¹¹ linear dynamic range	Up to 4000 channels, 10¹¹ linear dynamic range
	Detector	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)	Avalanche photodiode (APD)
	Intensity control	0.0001% – 100%, manual or automatic	0.0001% – 100%, manual or automatic	0.0001% – 100%, manual or automatic	0.0001% – 100%, manual or automatic	0.0001% – 100%, manual or automatic	0.0001% – 100%, manual or automatic	0.0001% – 100%, manual or automatic
	Dimensions (L x W x H)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)	62.5 x 40 x 24.5 cm (22 kg)
	Power supply	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A	AC 100-240 V, 50-60 Hz, 4A
	Conformity to standards	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099	21 CFR Part 11, ISO 13321, ISO 22412, ISO 13099
Optional Accessories	Disposable micro-volume cuvette	40 – 50 μL	40 – 50 μL	40 – 50 μL	N/A	40 – 50 μL	40 – 50 μL	40 – 50 μL
	Micro-volume glass cuvette	25 μL	N/A	25 μL	N/A	25 μL	N/A	25 μL
	Glass cuvette with round opening	1 mL	1 mL	1 mL	N/A	1 mL	1 mL	1 mL
	Capillary sizing cell	3 – 5 μL	N/A	3 – 5 μL	N/A	3 – 5 μL	N/A	3 – 5 μL
	Dip cell kit	1 – 1.5 mL, zeta potential measurement for organic-based samples	1 – 1.5 mL, zeta potential measurement for organic-based samples	1 – 1.5 mL, zeta potential measurement for organic-based samples	1 – 1.5 mL, zeta potential measurement for organic-based samples	N/A	N/A	N/A
* Dependent on samples and accessories † Up to 40% w/v using capillary sizing cell # 10mW 633nm He-Ne laser available on request

Not sure if it’s the right instrument?

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ViewSizer 3000

Written by Sophie Lindsey on February 2, 2024. Posted in Products.

Horiba Scientific
ViewSizer 3000

Simultaneous Multi-Laser Nanoparticle Tracking Analysis (NTA)

Measurement range 10 nm – 15 µm
Concentration Measurement Range: 1E5 to 1E9 particles/mL
No cross-contamination

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$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

Exosomes? Virus? Nanoparticle? Use multiple lasers for complete, detailed analysis of all the particles in your sample.

Exosomes, viruses, and nanoparticles all have wide size distributions which defeat traditional Nanoparticle Tracking Analysis (NTA) analyzers. The ViewSizer 3000 features simultaneous measurement with three lasers to collect the most accurate distribution and concentration information over a wide range of sizes within the same sample. Where the signal from a particle is too bright and saturates the detector from one laser, the software automatically uses data from a lower power laser to ensure the most accurate size and concentration information. On the other hand, when scattering from one laser is too weak for detection, the software uses data from a higher power laser to accurately track the particle.

Cross contamination is a concern in all analyses. Simplified cleaning means thorough cleaning. The easy-to-remove sample cells can be dissassembled for rapid, thorough cleaning, which leads to better data.

Escape the limits of traditional Nanoparticle Tracking Analysis

Accurate and sensitive analysis without cross contamination

Applications

Vaccine Manufacturing

Infectious Titer

Emulsion Polymerisation

Whiskey

Powder Metallurgy

Protein Aggregation

Exosomes

Protein Therapeutics

Drinking Water

Extracellular Vesicles

Features

The ViewSizer 3000 uses the latest advances in nanoparticle tracking analysis (NTA) to accurately determine particle properties!

Size Measurement Range: 10 nm to 15 microns depending on sample

The ViewSizer determines particle size and size distribution details by multi-laser nanoparticle tracking analysis (NTA). Multiple lasers enable analysis of a broad range of sizes in the same sample.

Concentration Measurement Range: 1E5 to 1E9 particles/mL

NTA can be used to count particles in the measurement volume. A patented method corrects for the effect of particle size on effective measurement volume.

No cross-contamination

The sample cell can be completely removed and dissassembled for complete cleaning. Disassembly, cleaning, and reassembly is faster than rinsing a flow cell. Furthermore, multiple cells can be used for faster throughput or assigned to various groups in a shared (core) facility.

Featured Publications for the ViewSizer 3000:

A lipase-independent pathway of lipid release and immune modulation by adipocytes	https://science.sciencemag.org/content/363/6430/989
Application of a novel new multispectral nanoparticle tracking technique	https://iopscience.iop.org/article/10.1088/1361-6501/aab940/meta
Biophysical characterization of polydisperse liposomal adjuvant formulations	https://doi.org/10.1016/j.bbrc.2020.05.156
Characterisation of particles in solution – a perspective on light scattering and comparative technologies	https://doi.org/10.1080/14686996.2018.1517587
Cyclodextrin Reduces Intravenous Toxicity of a Model Compound	https://doi.org/10.1016/j.xphs.2019.01.004
Development and anti-Candida evaluation of the vaginal delivery system of … nanosuspension-loaded thermogel	https://doi.org/10.1080/1061186X.2018.1434660
Electrochemical sensor based on F,N-doped carbon dots decorated laccase for detection of catechol	https://doi.org/10.1016/j.jelechem.2019.03.071
Light scattering by pure water and seawater: the depolarization ratio and its variation with salinity	https://doi.org/10.1364/AO.58.000991
Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis	https://doi.org/10.1016/j.ymthe.2018.05.014
Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells	https://doi.org/10.1038/s41598-020-67039-w
Multifunctional Nanocomposites Based on Liposomes and Layered Double Hydroxides Conjugated with Glycylsarcosine for Efficient Topical Drug Delivery to the Posterior Segment of the Eye	https://doi.org/10.1021/acs.molpharmaceut.8b01136
Particle size analysis of polydisperse liposome formulations with a novel multispectral advanced nanoparticle tracking technology	https://doi.org/10.1016/j.ijpharm.2019.06.013
Review of nanoparticles in ultrapure water: definitions and current metrologies for detection and control	https://www.ovivowater.com/review-of-nanoparticles-in-ultrapure-water-definitions-and-current-metrologies-for-detection-and-control/
Spark erosion as a high-throughput method for producing bimodal nanostructured 316L stainless steel powder	https://doi.org/10.1016/j.powtec.2018.01.012
Synthesis and Characterization of EGFR-Targeted Immunoporphysomes	http://hdl.handle.net/1807/89548
Synthesis of Ultrasmall Synthetic Melanin Nanoparticles by UV Irradiation in Acidic and Neutral Conditions	https://pubs.acs.org/doi/abs/10.1021/acsabm.9b00747
Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles \| Protocol (jove.com)	https://doi.org/10.3791/62447
Isolation and characterization of EV in Saliva of Children with Asthma	https://evcna.com/article/view/3962
Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential	https://doi.org/10.1016/j.bbi.2020.12.007
Skeletal muscle tissue secretes more extracellular vesicles than white adipose tissue and myofibers are a major source ex vivo but not in vivo	https://doi.org/10.1101/2020.09.27.313932
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands	https://www.nature.com/articles/s41598-021-84809-2
Purification of Cas9 – RNA Complexes by Ultrafiltration	https://doi.org/10.1002/btpr.3104
Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications	https://doi.org/10.1002/smtd.202100785
Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration	https://doi.org/10.1172/jci.insight.155928
Circulating Extracellular Vesicles From Septic Mice Induce Brain Inflammation via Mirna Cargo	http://hdl.handle.net/10713/19055

Specifications

Measurement Range	10 nm to 15 μm
Typical Sample Volume	350 µL to 2.5 mL
Typical Sample Concentration (Sample dependent)	10^5 – 10^9 particles/mL/p>
Sample Temperature Range (Controlled)	10° C to 50° C +/- 0.1° C
Dimensions	55 cm W x 66 cm D x 35 cm H
Weight	27 kg
Operational Environment	15° C to 30° C with < 85% RH

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Moxi V

Written by Sophie Lindsey on January 31, 2024. Posted in Products.

Orflo
Moxi V

Automated Cell Counter

True Cell Viability Counts
Precision Sizing
Highly Accurate
Rapid Assays

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$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

Gold Standard Automated Cell Counter, Cell Size, and Viability

The Orflo Moxi V is your top choice for an Automated Cell Counter due to its exceptional accuracy and efficiency. Utilising Coulter Principle and fluorescence-based technology, it provides precise, reliable results quickly. Its user-friendly interface and high-throughput capability streamline operations, making it ideal for research. With its compact design and robust performance, the Moxi V ensures consistent, high-quality cell analysis, making it a valuable addition to any laboratory.

Overview
The Moxi V Automated Cell Counter provides a combination of volumetric cell sizing (Particle Sizer) with simultaneous fluorescence (Cell Analyzer) to provide the most accurate cell counts, size, and viability in the industry. Specifically, the Moxi V employs the Coulter Principle to precisely measure the volumetric particle size of each particle for exact size measurements down to 3um in diameter (14fL volume), easily distinguishing between cells and debris. The system is also equipped with a 532nm laser and a 561nm/LP detection channel for robust cell viability analysis. Propidium Iodide (PI)-stained dead cells measure 50-100 times brighter fluorescence on the system than do live cells, removing the ambiguity associated with traditional Trypan Blue viability assessments. For each test, these size and viability measurements are applied, individually, to up to 23,000 cells in a matter of a few seconds. This ensures the highest level of precision and statistical robustness. As the fluidic volume is precisely metered as well, the particle counts are presented as an exact cell concentration.

The Moxi V employs a patented, single-use, microfluidic flow cell. The flow cells eliminate the hassle of traditional Cell Analyzers and Coulter Counters, eliminating the need for cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes. The Moxi V uses very little sample volume, 60ul, allowing you to conserve your precious, potentially expensive, sample (e.g. stem cells). Cell concentrations as low as 10 cells/ul are possible, typically requiring just 5ul of cell sample diluted in 55ul of PBS.

The Moxi V™ system comes standard with an ultra-intuitive, plug-and-play interface with free OS updates for as long as you own the instrument. No prior Cell Analysis experience is required – you simply just plug and play.

Some Key Features of the Moxi V include:
- True Cell Viability Counts – 50-100x more sensitive than vision counters.
- Precision Sizing – Uses the Coulter Principle to get precise cell volumes with CVs less than 3% and no need for triplicates.
- Highly Accurate – Accurate counts for smaller cells down to 3um (i.e. nuclei, RBCs). Uniquely accurate at low cell concentrations down to 10 cells per ul.
- Rapid Assays – Offers a less than 15 second test that counts up to 23,000 cells compared to 200-300 counts on most vision counters.

Technical Specs

Id	MXV102
Included accessories	USB power cord, US style USB power adapter, and Type S+ cassette pack
AC power type	110 VAC
Applications	Gold Standard Cell Count, Cell Size, and Viability
Battery Type	Rechargeable 3.7 V, 7500 mAh lithium ion
Cassette types	Type S+
Cell Particle Concentration Range	10,000 – 5,000 cells/mL Type S+
Cell types tested	HEK-293 HeLa PC12 CD3+T CHO-K1 Cos-7 HepG2 Hybridoma Jurkat E6-1 MCF7 Mesenchymal SC Monocyte Mouse ESC NIH 3T3 PBMC (cultured) Red Blood Cells (RBC) L5178y C. albicans (Yeast) S. cerevisiae Vin 13 (Yeast) S. cerevisiae X5 (Yeast) Wine Yeast (natural fermentaion) S.cerevisiae (Baker’s Yeast Safale US-05 Yeast
Data output formats	FCS 3.1, screen shots (.bmp), CSV
Data storage capasity	4Gb
Display resolution	800 x 480 colour touchscreen
Excitation wavelengths	532nm
In British units	8 lbs
Intended use statement	For Research Use Only. Product is not for use in diagnostic procedures
Laser colour	Green
Measurable dynamic range	3 – 27 microns Type Type S+ 4 – 35 microns Type MF-M
Measurement time	10 seconds Type S+
MPI cell health ratio	Yes (Size histogram only)
Number of detection channels flow parameters	2 colour, 1 size, 1 forward extinction
Number of PMTs	1
Optical detection range	561nm/LP (e.g. PI)
Particle size detection method	Impedimetric (Coulter Principle)
Pre-programmed tests	Gold Standard Cell Count, Cell Size, and Viability
Sample type	Cell Preparations
Sample volume	60 µL
Supported connectivity	USB on-the-go
Useable cell volume	14 – 10,306 fL Type S+ 14 – 22,449 fL Type MF-M
Weight	3.6 kg

How it works

The operating principle behind the Moxi V is a unique combination of Coulter-Principle cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser with a 561nm/LP (e.g. PI) emission filter. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is automatically performed by the system but can be easily adjusted/tuned by the user. The resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analysed.

Step 1:
Select desired app, insert the cassette and close the doors.

Step 2:
Once auto-alignment is complete, open the top door and pipette 60μl of labelled sample into the cassette.

Step 3:
Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi V, and load Sample 2.

Data

Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis package. The actual Moxi V screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi V and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.

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Moxi GO II

Written by Sophie Lindsey on January 30, 2024. Posted in Products.

Orflo
Moxi GO II

Mini Automated Cell Analyzer

Small footprint enables portability and convenience.
Fast (<10 sec) test times and no warm-up periods ensure rapid results.
Intuitive, touchscreen design for ease of use.
No cleaning or maintenance required.

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$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

Next Generation Coulter-Principle Cell Analyser.

The Orflo Moxi GO II Cell Analyser is the best solution for cell counting due to its precision, speed, and ease of use. Combining Coulter Principle-based sizing with fluorescence-based viability, it offers highly accurate and reproducible results. Its intuitive interface and automated processes ensure rapid and user-friendly operation, making it ideal for both novice and experienced users. The Moxi GO II’s compact design and high-throughput capabilities make it perfect for various applications, including research labs and biotech industries, providing consistent and reliable cell analysis.

Overview

Combines the Coulter Principle (for highly-accurate cell counts and exact, volumetric cell sizing) with a 488nm laser and two PMT fluorescence detection channels (for cell health assays, robust CAR-T monitoring, cellular response profiling, and immuno-profiling).

The Moxi GO II’s new Auto-Gating feature will analyse results in an accurate, repeatable way to provide the most consistent results — presented in a simplified new Data Summary page. The new Batch Mode feature allows you to run multiple tests of the same sample type. It will auto-find live and dead cell populations, eliminating user-to-user variability.

Technical Specs

Id	MXG102
Included accessories	USB power cord, US style USB power adapter, and Type S+ cassette pack
AC power type	110 VAC
Applications	Mulitplexed Bead ELISA’s In Cell Westerrns In Cell Protein Quant GFP Gold Standard Cell Count and Viability Mito Potential ROS Phagocytosis
Battery Type	Rechargeable 3.7 V, 4400 mAh lithium ion
Cassette types	Type S+
Cell Particle Concentration Range	5,000 – 1,000,000 cells/mL Type S+
Cell types tested	HEK-293 HeLa PC12 CD3+T CHO-K1 Cos-7 HepG2 Hybridoma Jurkat E6-1 K562 MCF7 Mesenchymal SC Monocyte Mouse ESC NIH 3T3 PBMC (cultured) Red Blood Cells (RBC) L5178y C. albicans (Yeast) S. cerevisiae Vin 13 (Yeast) S. cerevisiae X5 (Yeast) Wine Yeast (natural fermentaion) S.cerevisiae (Baker’s Yeast Safale US-05 Yeast
Data output formats	FCS 3.1, screen shots (.bmp), CSV
Data storage capasity	4Gb
Display resolution	800 x 480 color touchscreen
Excitation wavelengths	488nm
In British units	10 lbs
Intended use statement	For Research Use Only. Product is not for use in diagnostic procedures
Laser colour	Blue
Measurable dynamic range	3 – 27 microns Type Type S+ 4 – 35 microns Type MF-M
Measurement time	10 seconds Type S+
MPI cell health ratio	Yes (Size histogram only)
Number of detection channels flow parameters	2 color, 1 size, 1 forward extinction
Number of PMTs	2
Optical detection range	525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP)
Particle size detection method	Impedimetric (Coulter Principle)
Platform	Open platform: 561nm/LP (PI, PE, DS Red, Sytox Orange, 7 AAD, Nile Red, Rhodamine Red, Sun Coast Yellow, PE/Cy5), 525/45nm (FITC, GFP, Alexa Fluor 488nm, Calcein)
Pre-programmed tests	Mulitplexed Bead ELISA’s In Cell Westerrns In Cell Protein Quant RFP Gold Standard Cell Count and Viability Mito Potential ROS Phagocytosis
Resolution histogram bins	1000
Sample type	Beads Cell Preparations
Sample volume	60 µL
Supported connectivity	USB on-the-go
Useable cell volume	14 – 10,306 fL Type S+ 14 – 22,449 fL Type MF-M
Weight	4.53 kg

How it works

The operating principle behind the Moxi GO II Cell Analysers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 488nm (MXG102) solid state diode laser with and with the following emission filters – 525/45nm (e.g. FITC, GFP, Alexa 488) and 561nm/LP (e.g. PE, RFP). Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analysed.

Step 1:
Select desired app, insert the cassette and close the doors.

Step 2:
Once auto-alignment is complete, open the top door and pipette 60μl of labelled sample into the cassette.

Step 3:
Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi GO, and load Sample 2.

Data

Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi GO and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.
Applications

Apoptosis Monitoring

Transfection Monitoring

Yeast Monitoring in Brewing

Immuno-phenotyping

Mitochondrial Membrane

Reactive Oxygen Species

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Moxi Z

Written by Sophie Lindsey on January 29, 2024. Posted in Products.

Orflo
Moxi Z

Mini Automated Cell Counter Kit

Produce repeatable cell counts with >95% accuracy in just 8 seconds
Improve downstream results with better quality control
Offers Precise Cell Counting
Assess cell culture health without reagents or dyes
Measure the smallest cells at very high concentrations

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$Beckman Coulter LS 13 320 XR Laser Diffraction Particle Size Analyser 21 CFR Part 11$

MOXI Z Mini Automated Cell Counter Kit – Coulter Principle Counts and Sizing.

The Orflo Moxi Z offers precise cell counting through its advanced Coulter Principle technology and fluorescence-based viability analysis. Its user-friendly interface ensures quick and accurate measurements, making it ideal for research and clinical settings, enhancing cell analysis efficiency with reliable, reproducible results for various applications.

For research Use Only. Not for use in diagnostic procedures.

Overview
Improve downstream results with better quality control.

Moxi Z is the only automated cell counter that combines the Coulter Principle typically used in high-end cell counters with a patented thin-film sensor technology to allow for highly accurate (> 95%) and repeatable particle counting and sizing for a broad range of cell types – from mammalian cells to cells as small as wine yeast and more. Since today’s workflows demand accurate quality control of samples, determining cell counts precisely has a significant impact on outcomes and downstream costs.

Produce repeatable, precise cell counting with >95% accuracy in just 8 seconds.

This ultra-small instrument uses patented microfluidic thin-film cassettes that enable automatic load and measure operation. The resulting single cell volumetric measurements are completely technique-independent. It provides the ideal alternative to the tedious manual counting associated with cytometers, or the inaccurate results associated with image-based automated cell counters (typical accuracies of 75-80%).

Assess cell culture health without reagents or dyes.

Moxi Z also automatically reports a unique cell health assessment index – MPI (Moxi Population Index) – without the need for reagents or dyes.MPI is a ratio of the cell population of interest relative to the entire particle distribution in that sample, factoring in dead cells that have shrunken or broken apart as well as other debris and contaminants in the sample. For monodisperse mammalian cultures this is closely correlated to the overall health of the sample.In the case of mixed cell populations, it’s provides an assessment of the relative fraction of the largest sized population in the sample. Unlike staining-based viability methods that focus on the uptake by dead cells and do not account for contaminants and other debris, MPI provides insight on the primary population of interest (based on size) and its relationship to ALL other particles in the sample.

Measure the smallest cells at very high concentrations.

Using the new Type S cassette, you can now accurately measure the smallest cells of any automated cell counter (down to 3um average diameter) at concentrations of up to 2.5e+6/ml. This means the new Type S cassette can measure Yeast (including wine yeast) and non-spherical particles down to 14 fL in volume.

Results in just 8 seconds
- Ultra-small and ultra-easy to use (no manual counting, no focusing of any kind)
- Complete, high-resolution cell size/count histogram
- Handles “de-aggregation” and coincidence events
- Post-processing/analysis of count distributions with user-adjustable regions/gates
- Assess cell health (MPI) without the need for reagents like Trypan blue

Technical Specs

Id	MXZ001
Included accessories	Instrument only
AC power type	110 VAC
Applications	Cell Counting \| Cell Size \| Cell Health
Average Cell Diameter Range	4 – 25 microns Type M \| 3 – 20 microns Type S
Battery Type	Rechargeable 3.7 V, 4400 mAh lithium ion
Cassette types	Type M \| Type S
CE Certification	Yes
Cell Particle Concentration Range	3,000 – 500,000 cells/mL Type M \| 3,000 – 1,750,000 cells/mL Type S
Cell types tested	HEK-293 HeLa PC12 CD3+T CHO-K1 Cos-7 HepG2 Hybridoma Jurkat E6-1 K562 MCF7 Mesenchymal SC Monocyte Mouse ESC NIH 3T3 PBMC (cultured) Red Blood Cells (RBC) L5178y C. albicans (Yeast) S. cerevisiae Vin 13 (Yeast) S. cerevisiae X5 (Yeast) Wine Yeast (natural fermentaion) S.cerevisiae (Baker’s Yeast Safale US-05 Yeast
Data output formats	.csv format (Orflo MoxiChart or Microsoft Excel)
In British units	1.5 lbs
Intended use statement	For Research Use Only. Product is not for use in diagnostic procedures
Measurable dynamic range	4- 34 microns Type M 3 -26 microns Type S
Measurement time	15 seconds Type S 10 seconds Type M
MPI cell health	Yes
Number of detection channels flow parameters	1
Overall dimensions	7.6 L x 4.3 W x 2.8 H (in)
Particle size detection method	Impedimetric (Coulter Principle)
Platform	No
Pre-programmed tests	None
Sample type	Mammalian Cells\|Large Yeast\|Large Algae\|Protozoa Type M Mammalian Cells\|Most Yeast\|Small Algae\|Small Protozoa Type S
Sample volume	75 µL
Supported connectivity	USB on-the-go; PC or Mac compatible; requires Windows XP, Windows Vista, Windows 7, or Mac OS X operating system
Useable cell volume	34 – 20,580 fL Type M 14 – 4,200 fL Type S
Weight	0.68 kg

How it works

Electronic current is passed through a small hole (Cell Sensing Zone) in a thin-film membrane. Cells flow substantially single file through the CSZ causing momentary increases in measured voltage that are directly proportional to cell or particle volume. Thousands of cells are measured during a single test and the size of each cell is plotted and saved in histogram format. Total volumetric counts are determined by precisely measuring the
volume of fluid being analysed.

Insert cassette into Moxi Z.

Pipette 75 μL sample into cassette. Touch Screen.

Finished! Automatic, hands-free measurement in just 8 seconds.

Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into MoxiZ, and load Sample 2.

Data

Data For each measurement, a complete histogram is displayed as well as cell count, average cell size and Moxi Population Index. The raw waveform from the most recent cell count, containing each cell spike, is also stored on the Moxi Z and can be both viewed on the Moxi Z and uploaded to the PC for additional analysis. The Moxi Z also performs sophisticated curve fitting analysis to more accurately determine cell counts in the event of overlapping populations of cells (or debris), significantly improving the accuracy of the volumetric counts produced by the Moxi Z. Data for 500 samples may be stored on the unit. All data can be transferred to a PC/Mac via Bluetooth. No special software is required for the PC since date files are Excel®-compatible. Sophisticated PC/Mac compatible analys software is included with each Moxi Z. Cell health may be assessed by comparing differences in histograms from culture to culture.

Instructional Videos
Applications

Blood Cells

Monitoring Cell Health

Algal Growth

Yeast Monitoring

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Send a sample

BeScan Lab

Written by Sophie Lindsey on November 12, 2022. Posted in Products.

Bettersize BeScan Lab

Stability Analyser

Particle range 0.01 to 1,000 μm
Non-destructive stability analysis
Quantification of destabilisations and study of kinetics

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BeScan Lab, the versatile, sensitive, and reliable stability analyser based on Static Multiple Light Scattering (SMLS) technology, is widely used in the formulation development and product quality control. It accommodates a wide range of sample concentrations up to 95% v/v and types such as emulsions, suspensions, and foams, with temperature scanning capabilities reaching up to 80 °C. BeScan Lab provides both qualitative analysis and quantification of destabilisation, helping you monitor long-term product stability and achieve optimal shelf life.

Features and Benefits

● Real stability analysis for dispersions with volume fraction up to 95%

● Particle size measurement range from 0.01 to 1,000 μm

● Non-destructive stability analysis: Non-contact, non-dilution, non-shearing

● 20 µm resolution data acquisition enables quicker sample stability observation than the naked eye

● Temperature control up to 80 °C to accelerate destabilisation

● Identification of various unstable phenomena: creaming, sedimentation, flocculation, coalescence, and phase separation

● Quantification of destabilisations and study of kinetics

Key Features
What BeScan Lab Provides?
- lnstability index (l_us)
- Mean particle size
- Hydrodynamic analysis
- Radar chart for regional l_us
- Temperature trend testing
- Particle migration rate
Why You Need It?

From Raw Materials to Finish

BeScan Lab plays a crucial role throughout the product lifecycle, supporting formulation, production, and pre-use stages. It enables formulation optimization, quality control during manufacturing, investigation into optimal transportation and storage conditions, and research on redispersibility

1. Research and development

Ensure excellent dispersibility and uniformity through raw material selection.

2. Production and quality control

Optimize production processes, including method, time, and temperature, to enhance efficiency.

3. Storage and transportation

Evaluate formulation stability under varying environmental conditions, observing destabilization, and predict shelf life.

4. Pre-use treatment

Study the reversibility of destabilization and compliance with usage standards.

Features & Benefits

Non-destructive stability analysis for various dispersions
- Non-contact, non-dilution, non-shearing
- Sample volume fraction up to 95%
- Particle size measurement range from 0.01 to 1,000 μm
Fast and direct stability measurement
- The high-performance LED and ultra-sensitive detectors, with a 20-micron scan step, allow real-time monitoring and capture of subtle variations 200 times faster than the naked eye
- Temperature control up to 80 °C to accelerate destabilization
Qualitative and quantitative stability results
- Identification of various unstable phenomena, such as creaming, sedimentation, flocculation, coalescence, and phase separation
- Quantification of destabilizations and study of kinetics
Advanced Measurement Principle

Static Multiple Light Scattering (SMLS) is employed to characterize the stability of dispersions. Within BeScan Lab, a setup comprising two detectors and an LED light source ascends along the sample cell to conduct sample scanning. In the case of concentrated samples, the backward detector is employed to detect backscattered signals, while for diluted samples, the forward detector is utilized to detect transmitted signals.

Versatile Applications
- Agrochemicals
Evaluate the stability of pesticide formulations to predict shelf life and ensure the consistent performance of suspension systems.
- Battery and Energy
Test the stability of electrode materials and electrolytes, crucial for enhancing battery performance and lifespan.
- Ceramics
Analyze the stability of ceramic slurries and monitor the stability of glazes and pigments, ensuring reliable production processes.
- Home and Personal Care
Ensure product stability in cosmetics, lotions, creams, and other formulations for reliable performance.
- Food and Beverage
Test the stability of food products, from milk to sauces, and assess the dispersibility of food powders to maintain product quality.
- Petrochemicals
Monitor and ensure the stability of oil products, providing critical insights into the long-term performance of lubricants and the behavior of polymers in oil.
- Pharmaceuticals
Conduct stability testing for medicinal formulations, assess long-term drug stability, and analyze biomacromolecule aggregation to ensure product efficacy.
- Paints, Coatings and Inks
Measure the stability of coatings and inks, and evaluate the dispersion of pigments and dyes for uniform product quality.
Technology
Static Multiple Light Scattering

Static Multiple Light Scattering (SMLS) is an optical technique used to directly characterize native concentrated liquid dispersions. This technique emits light into the sample, where it is scattered multiple times by particles or droplets before being detected.

BeScan Lab applies SMLS using an 850 nm LED as light source, with detectors set at 0° for capturing transmitted light and at 135° for backscattered light. This setup scans the sample vertically, analyzing the transmitted light for transparent systems, while the backscattered light is ideal for opaque systems.

The signals are collected at 20 μm intervals, which enables precise observation of changes in size (d) and concentration (Φ) of suspended materials.

Signal display

Customized scanning procedures allow presentation of scans with different colors corresponding to different scanning times. The overlap of scans demonstrates how signals diverge from the reference as they vary with height and time. Intuitively, the scans capture local changes that characterize unstable phenomena.

The example illustrates that during sedimentation, the backscattered signals (dBS) undergo a distinctive pattern of change: a decrease at the top and an increase at the bottom, which is attributed to the migration of particles.

Features
- Versatile measurement
No limitations on color or viscosity, and suitable for a wide range of samples from low to high concentrations (up to 95% v/v).
- Non-destructive and in situ
Measures without preparation, thus preserving the sample’s original characteristics.
- Wide particle size range
Capable of measuring particles from 0.01 to 1,000 μm.
- Applicable to various systems
Suitable for emulsions, suspensions, foams, and other dispersions, providing robust and high-resolution measurements.
Software
Dedicated Software

for Superior Qualitative and Quantitative Stability Outcomes

Qualitative Analysis – Identification of Destabilisation

BeScan Lab utilises near-infrared light and a precise 20-micrometer spatial resolution to detect early-stage destabilisation phenomena like phase separation, sedimentation, creaming and aggregation (flocculation, coalescence, and coagulation) well before they are visually observable.

1. Flocculation often results in uniform changes in transmitted or backscattered signals across the entire sample height.
- Common in wastewater treatment, electrode slurries, and drilling fluids.
2. Phase separation typically involves evolving interfaces between phases over time.
- Common in paints and coatings, cosmetics.
3. Sedimentation causes a decrease in backscattered signals at the top and an increase at the bottom in opaque samples.
- Common in slurries, pigments, pesticides, vaccines, and body lotions.
4. Creaming in opaque samples enhances backscattered signals while lowering bottom signals.
- Common in milk-based beverages, lipid emulsions, and pesticides.
Quantitative Analysis – Instability Index for Rating Guide

BeScan Lab provides the instability index (I_US), which quantifies the stability of dispersions. The calculation involves summing all signal variations across the entire sample height and over time, capturing all subtle variations within the sample. This facilitates sample comparison, as a greater instability index (I_US) indicates lower stability. An instability index is automatically calculated after every scan using the following formula:

BeScan Lab offers instability indices over time to compare the stability of different samples. A slower increase in the instability index indicates higher dispersion stability, resulting in a flatter curve. Analysing the trend allows for predicting long-term stability.

Time-dependent instability index

1. Phase separation dynamics and mean particle size

Hydrodynamic analysis reveals layer thickness and particle migration rate over time, thereby determining the hydrodynamic mean diameter.

2. Optical analysis and mean particle size variation

Particle size variation analysis is achievable with BeScan Lab, correlating transmitted and backscattered light signals.

3. Temperature trend measurement

Programmable temperature trend measurement up to 80°C, which explores stability under extreme conditions and accelerates destabilisation.

4. Radar chart

Global and regional instability indices for each scanning are illustrated in form of a radar chart, intuitively providing a way to investigate regional stability (top, middle, and bottom).

Specification

Parameters	Values
Measurement principle	SMLS (Static Multiple Light Scattering)
Detection angle	0° transmission and 135° backscattering
Light source	850 nm LED
Scan step	20 μm
Scan height	0 – 60 mm
Number of samples	1
Maximum volume fraction*	95%
Measurement range of particle size	0.01 – 1000 μm
Temperature range	RT – 80 ℃ (±0.5 ℃ )
Sample volume	4 – 25 mL
Measurement mode	Regular/Fixed point/Temp. trend
Dimension	460(L) x 260(W) x 280(H) mm
Weight	13.5 kg
Power	AC100 – 240 V, 50 – 60 Hz, 3.8 A
ISO compliance	ISO/TR 18811:2018, ISO/TR 13097:2013 ISO/TR 21357:2022, ISO/TS 22107:2021

* Sample and sample preparation dependent

Applications

3D Printing Slurry

Detergent

Dispersant

Electrode Slurry

Ibuprofen

Pestiside

Beer Foam

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Tag: Particle Size Analysis

Bettersize InstrumentsDeepSizer 300

Submersible Particle Size Analyser

Safer In-situ Measurement

Real-time Sediment Monitoring

Accurate Results in High Turbidity

Reliable Long-term Field Deployment

Key Features

Technical Specs

Applications

Extreme Hydrological Conditions

Suspended Sediment Concentration

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Beckman Coulter LS 13 320 XR

Laser Diffraction Particle Size Analyser

Beckman Coulter LS 13 320 XR Particle Size Analyser

Key Features

Spot Small Differences

Easy-to-use Software

ADAPT Software enables 21 CFR Part 11

PIDS Technology* for Direct Detection of 10 nm Particles

Technical Specs

Accessories

Dry Powder System

Universal Liquid Module

Sonicator Control Unit

EU Vacuum Cleaner

Applications

Soils

Nanocellulose

Pigments

Cement

Capabilities

Using PIDS Technology

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Beckman CoulterMultisizer 4e

Coulter Coulter – Particle Size and Count Analyser

Accurate particle and cell counting

Key Features

Versatility

Sample Detection

Rapid Particle Counting

Data Management

Technical Specs

Applications

Yeast

Beer

Protein

E. coli

Plant Cells

CAR-T Cells

Capabilities

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BettersizeBettersizer S3 Plus

Particle Size and Shape Analyser

Features and Benefits

Performance

1) High Resolution and Sensitivity

2) Multiple Shape Parameters

3) Oversized Particle Detection

4) Analysis of Samples with Extremely Broad Distributions

Technology

Image Analysis

Laser Diffraction

Applications

Soy Milk

Advanced Ceramic Products

Powder Coatings

Ceramic Agglomerates

Lithium-Ion Batteries

Soils and Sediments

Abrasives

3D Printing

Differing Abrasives

Specification

Accessories

Introduction

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DeepSizer 300

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Measurement

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LS 13 320 XR

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Multisizer 4e

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Bettersizer S3 Plus

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Bettersizer 2600 Plus

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Bettersizer ST

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BeNano Series